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In Situ staining with Dextramer reagents combined with immunohistochemistry offers the possibility to assess questions like:
- where in the tissue compartment do the antigen-specific T cells accumulate?
- Do the antigen-specific T cells migrate through mucosa?
- Are there a subset of antigen-specific T cells particularly abundant in certain conditions?
- Are there any associations between subsets of antigen-specific T cells found in the blood and in the locale immune microenvironment?
read more about Dextramer reagents for in situ staining here