Immudex SARS-CoV-2 Packages

Reliable Identification of SARS-CoV-2-Specific T-Cell Responses

A deeper understanding of COVID-19 immunity is needed to explore vaccine responses, long-term immunity, predict patients at risk, and even prepare ourselves for a possible new pandemic in the future.

Our unique immune monitoring tools can help you unravel the SARS-CoV-2 specific T-cell immunity in an easy and reproducible way.

We provide four SARS-CoV-2 Packages, each with a curated collection of SARS-CoV-2-specific Dextramer® reagents displaying validated T-cell epitopes. Select the one most suitable for your research:

  • SARS-CoV-2 Spike Package
  • SARS-CoV-2 A*0201 Package
  • SARS-CoV-2 Multi Allele Package
  • SARS-CoV-2 Custom Package

Each SARS-CoV-2 Package is provided in two different sizes:

XL: Large package with carefully selected epitopes with broadest patients coverage.
S: Small package containing a selection of reagents displaying Top 5 or Top 10 T-cell epitopes.

The packages are available as:
MHC Dextramer®  - for identification of antigen-specific T cells in blood by flow cytometry
dCODE Dextramer® (HiT) - for bulk analysis of antigen-specific T cells by PCR and NGS
dCODE Dextramer® (10x compatible) -  for single-cell Multiomic analysis of antigen-specific T cells to combine information on TCR recognition, TCR sequencing, and gene expression using 10x Chromium Single Cell analysis.

SARS-CoV-2 Spike Package

Spike proteins of SARS-CoV-2 play a key role in the receptor recognition and cell membrane fusion process. Many COVID-19 vaccine development programs focus on the spike protein.

Immudex has prepared two curated Dextramer® Packages displaying proven T-cell epitopes from the Spike protein to help researchers evaluate their vaccine candidate's ability to elicit long-term SARS-CoV-2 T-cell immunity.

XL Package

  • Include T-cell epitopes from Spike protein shown to identify virus-specific T-cells in patients in multiple publications. Only epitopes identified in patient cohorts larger than 7 patients. All validated with MHC Multimer studies

S Package

  • Include Top 5 T-cell epitopes from Spike protein shown to identify virus-specific T-cells in patients in multiple publications

Allele

Peptide

SARS-CoV-2 Antigen

XL Package

S Package

A*0101

LTDEMIAQY

Spike

X

X

A*0201

VLNDILSRL

Spike

X

 

A*0201

YLQPRTFLL

Spike

X

X

A*0201

RLNEVAKNL

Spike

X

 

A*0201

NLNESLIDL

Spike

X

 

A*0201

FIAGLIAIV

Spike

X

 

A*0301

KCYGVSPTK

Spike

X

X

A*0301

GVYFASTEK

Spike

X

 

A*1101

RLFRKSNLK

Spike

X

 

A*1101

GVYFASTEK

Spike

X

 

A*2402

QYIKWPWYI

Spike

X

X

B*0702

SPRRARSVA

Spike

X

X

A*0201

ALIAPVHAV

Negative control

X

X

B*0801

AAKGRGAAL

Negative control

X

X

Reported patients coverage

10 - 100% in allele-matched subjects

More than 50% in allele-matched subjects

Total number of reagents

14

7

SARS-CoV-2 A*0201 Package

HLA-A*0201 is the most common HLA I allele in the European and North American populations.

Immudex has prepared two curated Dextramer® Packages displaying proven SARS-CoV-2 T-cell epitopes restricted by HLA-A*0201 for researchers to measure SARS-CoV-2 specific T cells and get a deeper understanding of the COVID-19 immunity.

XL Package

  • Include the most well-defined A*0201 restricted epitopes from 7 SARS-CoV-2 antigens, shown to identify virus-specific T-cells in patients in multiple publications. All validated with MHC Multimer studies

S Package

  • Include the top 5 most immune dominant epitopes from XL panel with high patient coverage in multiple publications

Allele

Peptide

SARS-CoV-2 Antigen

XL Package

S  Package

A*0201

YLQPRTFLL

Spike

X

X

A*0201

RLNEVAKNL

Spike

X

 

A*0201

RTIKVFTTV

ORF1ab

X

 

A*0201

TLACFVLAAV

Membrane

X

X

A*0201

ILLNKHIDA

Nucleocapsid

X

 

A*0201

NLNESLIDL

Spike

X

 

A*0201

FIAGLIAIV

Spike

X

X

A*0201

ALSKGVHFV

ORF3a

X

 

A*0201

KLWAQCVQL

ORF1ab

X

 

A*0201

YLFDESGEFKL

ORF1ab

X

 

A*0201

ALWEIQQVV

ORF1ab

X

 

A*0201

LLYDANYFL

ORF3a

X

X

A*0201

LLLDRLNQL

Nucleocapsid

X

X

A*0201

SLVKPSFYV

Envelope

X

 

A*0201

KLLEQWNLV

Membrane

X

 

A*0201

HLVDFQVTI

ORF6

X

 

A*0201

TLDSKTQSL

Spike

X

 

A*0201

VLNDILSRL

Spike

X

 

A*0201

FLLPSLATV

ORF1ab

X

 

A*0201

ILFTRFFYV

ORF1ab

X

 

A*0201

GMSRIGMEV

Nucleocapsid

X

 

A*0201

ALIAPVHAV

Negative control

x

X

Reported patients coverage

5-100% in allele matched subjects

10-100% in allele matched subjects

Total number of reagents

22

6

SARS-CoV-2 Multi Allele Package

Immudex keeps a curated and updated list of the published SARS-CoV-2 epitopes shown to be recognized by T cells in COVID-19 patients. We have created two curated Dextramer® Packages comprising the most immune dominant SARS-CoV-2 T-cell epitopes published in at least two publications.

With these two unique Packages, you get access to measure SARS-CoV-2 T-cell responses in a broad patient cohort following infections or vaccinations.

XL Package

  • Include T-cell epitopes shown to identity virus-specific T cells in patients in multiple publications. Covers 4 SARS-CoV-2 Antigens and 6 HLA Alleles

S Package

  • Include the most immune dominant T-cell epitope(s) for each allele and is a subset of the XL panel. Covers 4 SARS-CoV-2 antigens and 6 HLA alleles. All have high patient coverage in multiple publications

Allele

Peptide

SARS-CoV-2 Antigen

XL Package

S Package

A*0101

CTDDNALAYY

ORF1ab

X

 

A*0101

DTDFVNEFY

ORF1ab

X

 

A*0101

FTSDYYQLY

ORF3a

X

X

A*0101

GTDLEGNFY

ORF1ab

X

 

A*0101

LTDEMIAQY

Spike

X

 

A*0101

PTDNYITTY

ORF1ab

X

 

A*0101

TTDPSFLGRY

ORF1ab

X

X

A*0201

ALSKGVHFV

ORF3a

X

 

A*0201

ALWEIQQVV

ORF1ab

X

 

A*0201

LLLDRLNQL

Nucleocapsid

X

 

A*0201

LLYDANYFL

ORF3a

X

X

A*0201

YLQPRTFLL

Spike

X

X

A*0301

KTFPPTEPK

Nucleocapsid

X

X

A*0301

KTIQPRVEK

ORF1ab

X

 

A*0301

VVYRGTTTYK

ORF1ab

X

 

A*1101

ASMPTTIAK

ORF1ab

X

 

A*1101

ATEGALNTPK

Nucleocapsid

X

X

A*1101

KTFPPTEPK

Nucleocapsid

X

X

A*1101

SAFAMMFVK

ORF1ab

X

 

A*1101

STFNVPMEK

ORF1ab

X

 

A*2402

QYIKWPWYI

Spike

X

X

A*2402

VYFLQSINF

ORF3a

X

X

A*2402

VYIGDPAQL

ORF1ab

X

 

B*0702

IPRRNVATL

ORF1ab

X

 

B*0702

KPRQKRTAT

Nucleocapsid

X

 

B*0702

SPRRARSVA

Spike

X

 

B*0702

SPRWYFYYL

Nucleocapsid

X

X

A*0201

ALIAPVHAV

Negative Control

X

X

B*0801

AAKGRGAAL

Negative Control

X

X

Reported patients coverage

10-100% in allele matched subjects

20-100% in allele matched subjects

Total number of reagents

29

12

SARS-CoV-2 Custom Package

We are well aware that not all patients cohorts are the same and that sometimes, you are the only one able to design the Panel most suitable for your research.

Choose your preferred SARS-CoV-2 specific reagents and build your customized package. Have a look at the continuously updated list of the published COVID-19 T-cell epitopes.

SARS-CoV-2 Custom Package
As many epitopes as you like 

  • Include:
    - T-cell epitopes from your own selection
    - T-cell epitopes from Immudex SARS-CoV-2 Packages or epitope list
  • Alleles: any MHC I or MHC II from our catalog

How to order your SARS-Cov-2 Package

Send an e-mail to ordering@immudex.com with the following information:

  • Catalog number of the selected package. Find it here: SARS-CoV-2 Package Overview
  • Your preferred dye (For MHC Dextramer®): PE, APC, FITC, or None
  • Test size: for MHC Dextramer®, select between 50 or 150 tests; for dCODE Dextramer®, select between 25, 50 or 150 tests.

If you are interested in the Custom Package send an email to ordering@immudex.com with the alleles and epitopes you have selected for your research. 

 

Download the SARS-CoV-2 Package Overview

Learn more about T-cell responses to SARS-CoV-2