Create Your Own Dextramer® Reagent
Klickmer technology allows design of custom Dextramer reagents, displaying ligands of choice. A Klickmer is a Dextramer backbone, carrying multiple acceptor sites for biotinylated molecules. Klickmer benefits of the superiority of the Dextramer technology, but gives the freedom to create customized, high-avidity multimer reagents.
"Any" Kind of Biotinylated Molecule or Cell Can Be Linked to Klickmer, Including:
- B-cell antigens
- Soluble TCRs
- Immune stimulatory molecules
- New fluorochromes
- Metal chelates/Lanthanides
Get Inspired by Klickmer:
This multipurpose tool serves a range of applications and can help you address new scientific questions:
- Build reagents for B-cell detection
- Identify ligands for low-affinity or orphan receptors
- Create an artificial vaccine scaffold
- Stimulate immune cells in vitro
Key Features of Klickmer:
Applications of Klickmer™ Technology
The flexibility of Klickmer technology serves a range of applications and can help address diverse needs in research and development.
Read here how the Klickmer technology has advanced different research areas.
Research Area: Cancer
Technique: Flow Cytometry
Klickmer Application: Multimerization of VISTA ligand
Reference: Johnston RJ et al., Nature. 2019, doi: 10.1038/s41586-019-1674-5
|Investigation of the effects of cancer environment acidity on T-cell dysfunction||
VISTA ligand is expressed by circulating and intratumoral myeloid cells and plays a role in inhibiting T-cell responses. The authors used Klickmer to multimerize VISTA protein. The resulting Dextramer® was used to detect interacting lymphocytes at different pH. The authors reported that VISTA engages and suppresses T-cells selectively at acidic pH such as tumor microenvironments.
Research Area:Vaccine Development
Technique: Macaques Immunization
Klickmer Application: Multimerization of viral proteins
Reference: Yang GB et al., J Gen Virol. 2012, doi: 10.1099/vir.0.041061-0
|Generation of a vaccine against simian-human immunodeficiency virus (SHIV) in macaques||Mamu I, HIV gp140, SIV p27, and HSP 70 proteins were multimerized, using Klickmer. This Dextramer was used to immunize macaques, prior to rectal challenge with SHIV. A significant decrease in viral load was observed in macaques immunized with the Dextramer-based vaccine.|
Research Area: Infectious Disease
Technique: Flow Cytometry
Klickmer Application: Multimerization of T-cell receptor
Reference: Chancellor A et al., Proc Natl Acad Sci U S A. 2017, doi: 10.1073/pnas.1708252114
|Investigation of the influence of structural variations of ligands lipids chain on the interaction with TCRs||The Germline-encoded mycolyl lipid T-cell receptor (GEM-TCR) interacts with Mycobacterium tuberculosis (Mtb) lipids presented by CD1b. The authors used Klickmer to multimerize GEM-TCR. The resulting Dextramer reagent was used to detect CD1b molecules loaded with Mtb lipids with different chain structures. This study showed that the interaction of GEM-TCR with lipid-loaded CD1b depends on the lipids' chain structure.|
Research Area: Transplantation
Klickmer Application: Multimerization of MHC I and MHC II molecules
Reference: Luque S et al., J Immunol Methods. 2018. doi: 10.1016/j.jim.2018.07.01
|Detection of circulating memory B-cells in transplanted patients||To quantify long-term antigen-specific B cells-driven humoral immunity in human transplantation, it is important to measure the levels of circulating antibodies as well as memory B cells (mBC). The authors used Klickmer to multimerize MHC I and II molecules. The Dextramer reagent was then applied to develop a refined B-cell ELISpot assay for the detection of mBCs.