Decoding Human CMV-Specific CD8+ T Cells Through Multi-Layer Single-Cell Omics
Webinar Recording
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This webinar presents a multi-layer single-cell approach for the identification and characterization of human antigen-specific CD8⁺ T cells. By combining DNA-barcoded pHLA multimers with transcriptomics, surface marker profiling, and TCR sequencing, the workflow links antigen specificity to cellular phenotype and clonality at single-cell resolution.
Key learning points:
- Antigen-specificity assignment from DNA-barcoded pHLA multimer data
- Flexible multiplexing strategies for single-cell studies
- Integration of transcriptomes, surface proteins, TCR repertoires, and antigen specificity
Speakers:
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Ioanna Gemünd, PhD | Postdoctoral Researcher, Institute of Virology, TU Munich (TUM)
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Thomas H. Blicher, PhD | Principle Scientist, Immudex