Visualize the Landscape of Tissue-Specific Immunity

Tracking T cells in the tissue where they exert their function can advance our understanding of T-cell immunity. MHC I Dextramer® for in-situ staining reagents can detect spatial distribution, localization, and abundance of antigen-specific T cells in different tissue sites in health and disease.

Antigen-specific T cells in tissue sections can be visualized by direct or indirect immunohistochemistry (IHC) methods. Direct visualization uses the conjugated fluorophores on the MHC I Dextramer® reagent, while indirect visualization uses antibody staining directed against the fluorophore on the MHC I Dextramer® for signal amplification. MHC I Dextramer® for in-situ staining reagents provides T-cell staining in fresh samples, paraffin-embedded (FFPE), and frozen sections.

Solid tissue like tumors and lymphoid organs have different structures and spatial distributions of infiltrating T cells, often challenging to identify.

Immudex offers the Dextramer® In-Situ Staining Kit to optimize T-cell staining in solid tissue sections by IHC and comprises three Dextramer® reagents where:

  • Each Dextramer® carries the same specificity (e.g., HLA-A*0201/ELAGIGILTV)
  • Each Dextramer® has a different number of MHC-peptide complexes, providing reagents with low, medium, or high avidity, respectively
  • Each Dextramer® is FITC labeled for direct visualization by fluorescent microscopy

Cryosections stained with CD8 antibody (green) and HLA-A2/HY Dextramer® (red). Yellow arrows indicate colothecalization of HLA-A2/HY Dextramer® and CD8 antibodies on the same cell.

Figure adapted from Kim YH, et al., 2012

Benefits of the Dextramer® In-Situ Staining Kit

By utilizing the Dextramer® In-Situ Staining Kit, you can identify the reagent optimal for your tissue type and experimental setup. Optimization may also be required for other steps of the IHC procedure, e.g., tissue preparation and antibodies.

Immudex manufactures Dextramer® for in-situ staining reagents made with MHC class I alleles, as listed in the table below. Customized Dextramer® for in-situ staining reagents are easily made with MHC alleles from our catalog and peptides forming stable complexes with the MHC molecule.

How to Order Dextramer® In-Situ Staining Kit

Send an e-mail to [email protected] specifying:

  • Catalog number or the MHC allele-peptide combination. Please check our current list of available MHC alleles
Content Amount Fluorophore Price (EUR) Price (USD)
Dextramer® In-Situ Staining 0,2 mL FITC 3.430 3,910
Dextramer® In-Situ Staining 1 mL FITC 8.620 9,845

How to Order Dextramer® for In-Situ Staining

Find your Dextramer® reagent in the table below, or send an e-mail to [email protected] specifying:

  • Cat. no or the MHC allele-peptide combination. Please check our current list of available MHC alleles
  • Fluorophore (PE, APC, or FITC) or no Fluorophore (None)
  • Test sizes (50, 150, 500, 1000 tests)

Immudex has updated the catalog numbers. To learn more about it, please consult the document here

Selected References

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Our webshop does not include all products. Contact us or email [email protected] and we will search our larger internal database of products for you. We also provide custom alleles and specificities.

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