Dextramer® for In-Situ Staining

Visualize the Landscape of Tissue-Specific Immunity

Tracking T cells in the tissue where they exert their function can advance our understanding of T-cell immunity. MHC I Dextramer® for in-situ staining reagents can detect spatial distribution, localization, and abundance of antigen-specific T cells in different tissue sites in health and disease.

Antigen-specific T cells in tissue sections can be visualized by direct or indirect immunohistochemistry (IHC) methods. Direct visualization uses the conjugated fluorophores on the MHC I Dextramer® reagent, while indirect visualization uses antibody staining directed against the fluorophore on the MHC I Dextramer® for signal amplification. MHC I Dextramer® for in-situ staining reagents provide T-cell staining in fresh samples, paraffin-embedded (FFPE), and frozen sections.

Solid tissue like tumors and lymphoid organs have different structures and spatial distributions of infiltrating T cells, often challenging to identify.

Immudex offers the Dextramer® In-Situ Staining Kit to optimize T-cell staining in solid tissue sections by IHC and comprises three Dextramer® reagents where:

  • Each Dextramer® carries the same specificity (e.g., HLA-A*0201/ELAGIGILTV)
  • Each Dextramer® has a different number of MHC-peptide complexes, providing reagents with low, medium, or high avidity, respectively
  • Each Dextramer® is FITC labeled for direct visualization by fluorescent microscopy

Benefits of the Dextramer® In-Situ Staining Kit

By utilizing the Dextramer® In-Situ Staining Kit, you can identify the reagent optimal for your tissue type and experimental setup. Optimization may also be required for other steps of the IHC procedure, e.g., tissue preparation and antibodies.

Immudex manufactures Dextramer® for in-situ staining reagents made with MHC class I alleles, as listed in the table below. Customized Dextramer® for in-situ staining reagents are easily made with MHC alleles from our catalog and peptides forming stable complexes with the MHC molecule.

How to Order Dextramer® In-Situ Staining Kit

Send an e-mail to ordering@immudex.com specifying:

  • Catalog number or the MHC allele-peptide combination. Please check our current list of available MHC alleles
Content Amount Fluorophore Price (EUR) Price (USD)
Dextramer® In-Situ Staining 0,2 mL FITC 3069 3499
Dextramer® In-Situ Staining 1 mL FITC 7719 8799

How to Order Dextramer® for In-Situ Staining

Find your Dextramer® reagent in the table below, or send an e-mail to ordering@immudex.com specifying:

  • Cat. no or the MHC allele-peptide combination. Please check our current list of available MHC alleles
  • Fluorophore (PE, APC, or FITC) or no Fluorophore (None)
  • Test sizes (50, 150, 500, 1000 tests)

Immudex has updated the catalog numbers. To learn more about it, please consult the document here

Selected References

  1. Farber DL, et al. Tissues, not blood, are where immune cells function. Nature 2021; 593, 506-509.
  2. Weisberg SP, et al. Tissue-specific immunity for a changing world. Cell 2021; 184(6): 1517 – 1529.
  3. Lasrado N, et al. Mechanisms of sex hormones in autoimmunity: focus on EAE. Biology of Sex Differences, 2020; 11:50.
  4. Mlecnick B, et al. Integrative analyses of colorectal cancer show immunoscore is a stronger predictor of patient survival than microsatellite instability. Immunity 2016; 44(3):698-711.
  5. Dolton G, et al. Comparison of peptide–major histocompatibility complex tetramers and dextramers for the identification of antigen-specific T cells. Clin & Exp Immunol 2014; 177: 47–63.
  6. Kim YH, et al. In situ detection of HY-specific T cells in acute graft-versus-host disease-affected male skin after sex-mismatched stem cell transplantation. Biol Blood Marrow Transplant. 2012; 18(3):381-387.
  7. Massilamany C, et al. Direct staining with major histocompatibility complex class II dextramers permits detection of antigen-specific, autoreactive CD4 T cells in situ. PLoS One 2014; 9(1):e87519.
  8. Massilamany C, et al. In situ detection of autoreactive CD4 T cells in brain and heart using major histocompatibility complex class II dextramers. J Vis Exp. 2014; (90):e51679.

Cryosections stained with CD8 antibody (green) and HLA-A2/HY Dextramer® (red). Yellow arrows indicate colocalization of HLA-A2/HY Dextramer® and CD8 antibodies on the same cell.

Figure adapted from Kim YH, et al., 2012

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