Monitor Cellular Immunity in Infectious Diseases

Define factors of protective immunity and identify effective vaccine antigens

 

Central to human immunity towards infectious diseases is the activation of pathogen-specific T cells. CD8+ and CD4+ T cells play a key role in mediating protective immunity after immune challenges such as infection or vaccination. Memory CD8+ or CD4+ T cells are retained after exposure to viral or bacterial antigens to sense reinfection or recurrence. These memory T cells are licensed to respond quickly, remain highly vigilant, and are capable of cytotoxicity.

In the lab, antigen-specific T cells can help us better understand the immune response mounted to infection to better understand disease progression or predict targets for vaccine development. Detection of cellular immunity can provide an insight into the patient’s general immune status before, during, and after immunotherapy. Furthermore, tracking pathogen-specific T cell presence reveals immune status and can anticipate the risk of severe disease progression in immunocompromised patients.

Solutions for Your Research Needs

Every day, immunologists make new discoveries that deepen our understanding of how the immune system responds to infectious diseases.. Learn how Dextramer® technology can help you detect, quantify, or isolate pathogen-specific CD8+ and CD4+ T cells in peripheral blood, cell cultures, or tissue across different platforms, allowing you to continue directly from in-situ, to flow cytometry, and move onto NGS or single-cell multi-omics:

  • MHC I and MHC II Dextramer® reagents comprise a broad selection of T-cell epitopes to monitor the whole spectrum of virus- or bacterial-specific T-cell responses by flow cytometry, including T cells with rare and low-affinity receptors.
  • MHC I Dextramer® In-Situ Staining reagents can help detect distinct localization, distribution, and abundance of virus- or bacterial-specific CD8+ T cells within diverse tissue niches in health and disease.
  • dCODE Dextramer® reagents for epitope discovery and T-cell profiling in bulk solution or at single-cell level. The unique DNA barcode enables the detection of antigen-specific T cells by PCR followed by next generation sequencing (NGS) and single-cell multi-omics.
  • U-Load Dextramer® reagents to easily make your choice of customized multimers directly in the lab from a broad range of peptide-receptive MHC I and MHC II alleles and your own peptide, via flow cytometry or single-cell multi-omics.
  • Klickmer® reagents can be customized to help you to explore immunity beyond T cells by looking into other immune cells like B cells or study TCR-ligand interactions in virus cells by flow cytometry or single-cell multi-omics.

Uncover antigen-specific immune cell responses and provide new insights in vaccine development

Capturing Primary and Secondary Anti-Yellow Fever Vaccine Response to Investigate the Dynamics of Antigen-Specific T Cells

CD8+ T-Cell signature in acute SARS-CoV-2 infection identifies memory precursors

Successful detection of influenza A-specific CD4+ T cells using the Klickmer® technology

Published Viral T-Cell Epitopes

To support clinicians and scientists worldwide in their virus research, we want to share curated lists of the human, mouse, and primate viral antigens recognized by CD8+ and CD4+ T cells. These lists of virus-specific T-cell epitopes reflect the product portfolio of Immudex and the research of many dedicated scientists published in peer-reviewed papers. You can now select the epitopes from the comprehensive lists that best fit your research within infectious disease or vaccine development.

Published Viral Human, Primate, and Mouse T-Cell Epitopes

Download the list of epitopes in viral infections

Published Human COVID-19 T-Cell Epitopes

Download the list of epitopes in COVID-19 infected or vaccinated individuals

Published Mouse COVID-19 T-Cell Epitopes

Download the list of epitopes in COVID-19 infected genetically modified mice

Dextramer® Panels

Our predefined products and Panels of Dextramer® reagents address highly relevant viral or bacterial infections. Alternatively, you can select antigens of interest from our epitope lists, product menu – or define custom Dextramer® reagents

Virus Dextramer® Panel

Measure immune status with selected Dextramer® reagents displaying epitopes of the opportunistic cytomegalovirus, Epstein-Barr virus, influenza, and BK virus.
Opportunistic viruses can cause severe diseases in immune-compromised patients. Measuring the virus immune status is an important tool to check the risk of infection in immune-compromised patients.

Order MHC Dextramer® Virus Panel

The MHC Dextramer® Virus Panel consists of Dextramer® reagents, displaying immunodominant, well-characterized epitopes derived from four general and opportunistic viruses: Cytomegalovirus (CMV), Epstein-Barr virus (EBV), Influenza, and BK virus and a negative control Dextramer®.

Cat. No. RX03 - Virus-specific Dextramer®

MHC Allele

Peptide

Virus Tests* 
HLA-A*0201 NLVPMVATV CMV 50 tests
HLA-A*0201 GLCTLVAML EBV 50 tests
HLA-A*0201 GILGFVFTL Influenza 50 tests
HLA-A*0201 LLMWEAVTV BK virus 50 tests
HLA-A*0201 Negative Control  - 50 tests

* One test is defined as 10 µl MHC Dextramer® reagent sufficient to stain up to 1-3 x 10^6 lymphoid cells or 1-3 x 10^5 clonal antigen-specific T cells.

Virus Dextramer® Panel is for research use only.

Immudex has updated the catalog numbers. To learn more about it, please consult the document here

COVID-19 Dextramer® Panels

A deeper understanding of COVID-19 immunity is needed to explore vaccine responses, long-term immunity, predict patients at risk, and even prepare ourselves for a possible new pandemic in the future.
Our unique immune monitoring tools can help you unravel the SARS-CoV-2 specific T-cell immunity in an easy and reproducible way.

Order COVID-19 Specific Dextramer® Products

Unravel T-cell response to SARS-CoV-2 infection with a dedicated set of Dextramer® products featuring T-cell epitopes validated in COVID-19 patients.

We provide four SARS-CoV-2 Panels, each with a curated collection of SARS-CoV-2-specific Dextramer® reagents displaying validated T-cell epitopes. Select the one most suitable for your research:

  • SARS-CoV-2 Spike Panel
  • SARS-CoV-2 A*0201 Panel
  • SARS-CoV-2 Multi Allele Panel
  • SARS-CoV-2 Custom Panel

For more details, please read here or contact us

CMV Dextramer® Panel

CMV infection and disease pose a major risk in immunosuppressed patients. Reactivation of CMV is a frequently occurring complication and can contribute to morbidity and mortality if not controlled. CMV-specific CD8+ T cells are considered the main factor in successful management of CMV infection. In transplant patients, a low number of CMV-specific CD8+ T cells are predictive for development of CMV-related complications.​

Support successful management of cytomegalovirus infection in transplant patients with well-characterized, virus-specific epitopes.

Order MHC Dextramer® CMV Panel

The MHC Dextramer® CMV Panel consists of Dextramer® reagents, displaying immunodominant, well-characterized epitopes derived from CMV-specific antigens and a negative control Dextramer®.

Cat. No. RX04 - CMV-specific Dextramer®

MHC Allele
Peptide Antigen Tests*
HLA-A*0101 VTEHDTLLY pp50 50 tests
HLA-A*0201 NLVPMVATV pp65 2 x 50 tests
HLA-A*0301 KLGGALQAK IE-1 50 tests
HLA-A*2402 QYDPVAALF pp65 50 tests
HLA-B*0702 RPHERNGFTVL pp65 50 tests
HLA-B*0702 TPRVTGGGAM pp65 50 tests
HLA-B*0801 ELRRKMMYM pp65 50 tests
HLA-B*3501 IPSINVHHY pp65 50 tests
General Negative Control  -  3 x 50 tests

* One test is defined as 10 µl MHC Dextramer® reagent sufficient to stain up to 1-3 x 10^6 lymphoid cells or 1-3 x 10^5 clonal antigen-specific T cells.

MHC Dextramer® CMV Panel is for research use only. For In Vitro Diagnostics (IVD) use please visit the Dextramer® CMV Kit product page.

Immudex has updated the catalog numbers. To learn more about it, please consult the document here

EBV Dextramer® Panel

EBV is a persistent virus that infects 90% of the human population. Primary infection may cause acute infectious mononucleosis, and persistent infection may be associated with the development of several malignancies. T cells play a critical role in controlling both the primary and persistent phases of infection.

Detect, quantify, and isolate EBV-specific T cells and obtain information about the patients’ virus immune status.

Order MHC Dextramer® EBV Panel

The panel consists of Dextramer® reagents displaying immunodominant, well-characterized epitopes derived from EBV-associated antigens and a negative control Dextramer.

Cat. No. RX05 - EBV-specific Dextramer®

MHC Allele

Peptide

Antigen Tests*
HLA-A*0201 GLCTLVAML BMLF-1 50 tests
HLA-B*3501  HPVGEADYFEY EBNA-1 50 tests
HLA-B*0702  RPPIFIRRL EBNA-3A 50 tests
HLA-B*0801  FLRGRAYGL EBNA-3A 50 tests
General Negative Control  - 50 tests

* One test is defined as 10 µl MHC Dextramer® reagent sufficient to stain up to 1-3 x 10^6 lymphoid cells or 1-3 x 10^5 clonal antigen-specific T cells.

The MHC Dextramer® EBV Panel is for research use only.

Immudex has updated the catalog numbers. To learn more about it, please consult the document here

Bacterial and Parasitic Disease Dextramer® Products

T cells are one of the cornerstones of the immunological response against infections caused by bacterial pathogens and parasitic organisms. Detection and monitoring of antigen-specific T cells is of great importance in studies of immune protection. Our immune monitoring tools can help you to investigate the T-cell response in diseases ranging from Tuberculosis (Mycobacterium tuberculosis) to Malaria (Plasmodium), Enterococcus faecium, and Lyme borreliosis (Borrelia burgdorferi).

Order Bacterial and Parasitic Disease Dextramer® Products​

Detect, quantify, and isolate antigen-specific T cells to support your studies of infectious diseases caused by bacterial pathogens or parasitic organisms.

Allele

Peptide

Antigen

Category

Cat. No.​

H-2 Kb

IMYNYPAM

TB10.3-4

TB

JD3326

H-2 Kd

GYETVITQL

PPE

TB

JE3561

H-2 Kk

VESTAGSL

ESAT-6

TB

JF3408

H-2 Ld

MPVGGQSSF

Ag85A

TB

JG3347

HLA-A*2402

AYQGVQQKV

ESAT-6

TB

WF3373

H-2 Kb

DAPIYTNV

Beta-gal

Bacterial

JD3592

H-2 Kb

ICPMYARV

Beta-gal

Bacterial

JD3591

H-2 Ld

TPHPARIGL

Beta-gal

Bacterial

JG3295

H-2 Kk

YENDIEKKI

PLAM csp

Parasite

JF2188

H-2 Kd

IYNVGQVSI

Sialidases 

Parasite

JE3441

H-2 Kk

TEWETGQI

ASP-2

Parasite

JF3263

H-2 Kk

DYENDIEKKI

CPS

Parasite

JF2181

For more details or assistance with ordering, please contact us.

CD1d Dextramer® Reagents are also available for the study of the immune response in Lyme borreliosis (Borrelia burgdorferi).

 

 

Accelerate Your Vaccine Development Research

See how dCODE Dextramer® technologies can advance all vaccine development phases, from selecting candidate viral epitopes to monitoring vaccine efficacy by measuring antigen-specific immunity.

Read More