MHC II Dextramer® – Identify Antigen-Specific CD4+ T Cells

Enhanced Sensitivity for Reliable Results

Typically expressed on the surface of antigen-presenting cells, MHC II molecules dock and primes CD4+ T cells.

Antigen-specific CD4+ T cells are critical for the immune response of many diseases, given their role in recruiting and coordinating other immune cells. Often challenging to detect and stratify with standard reagents, antigen-specific CD4+ T cells are revealed through the high-avidity and enhanced sensitivity of the Dextramer® technology.

With multiple MHC-peptide complexes and fluorophores on a flexible dextran backbone, MHC II Dextramer® reagents are designed for high-resolution characterization of CD4+ T-cell responses, even with low-abundance cells and in complex matrices like blood.

MHC II Dextramer® detects DRB1*0101/EBV-specific T cells in a blood sample via flow cytometry (internal data).

A Tool For Meticulous And Precise Research

Push the boundaries of your flow cytometry assays with MHC II Dextramer® reagents. Designed for high avidity and exceptional signal-to-noise ratio, MHC II Dextramer® reagents enable you to detect antigen-specific CD4+ T cells with confidence.

• Get the full picture of CD4+ T-cell responses, even the low-affinity ones.
• Ensure consistent, reproducible, and comparable results.
• Expand the limits of your research.
• Secure flexibility in your experiments.

Order MHC II Dextramer®

You can order MHC II Dextramer® reagents in one of three ways:

1. Use the table below to find and order your MHC II Dextramer® reagent
2. If you can’t find the reagent to meet your research needs, create a custom reagent
3. Alternatively, send an e-mail to ordering@immudex.com specifying:

• Catalog number or desired MHC allele-peptide combination.
       - Consult our lists of available MHC alleles
       - Calculate the predicted MHC-peptide affinity using our prediction tool
• Fluorophore (PE, APC, FITC, or none)
• Test size (50,150, 500, 1000 tests). One test is 10 µl reagent and sufficient to stain 1–3 x 10^6 lymphoid cells or 1–3 x 10^5 clonal antigen-specific T cells.

Immudex has updated the catalog numbers. To learn more about it, please consult the document here

* For customized products an entry fee of 550EUR/ 650USD is added to evaluate each unique peptide's ability to bind to the requested MHC II allele using standardized peptide binding assays.