MHC II Dextramer®

Identify Antigen-specific CD4+ T Cells

Antigen-specific CD4+ T cells play an important role in the immune response of autoimmune disease, cancer, transplantation, infectious disease, and allergy.

MHC II Dextramer® reagents can identify antigen-specific CD4+ T-cell subsets by flow cytometry.

MHC II Dextramer reagents consist of a protein-stabilizing dextran backbone, an optimized number of MHC-peptide complexes, and fluorophores (PE, APC or FITC) or no fluorophore (None).

Immudex manufactures MHC II Dextramer reagents as listed in the table below. Customized reagents are easily made with MHC alleles from our catalog and with peptides that form stable complexes with the MHC molecule. 

Detection of DRB1*0101/EBV-specific T cells in blood sample using flow cytometry.

How to Order MHC II Dextramer®

Please send an e-mail to ordering@immudex.com specifying:

  • Cat. no or the MHC allele-peptide combination of the MHC II Dextramer reagent.
    See our current list of MHC alleles
  • Fluorophore (PE, APC or FITC) or no Fluorophore (None)
  • Test sizes (50, 150, 500, 1000 tests). One test is defined as 10 µl MHC Dextramer reagent, sufficient to stain up to 1-3 x 10^6 lymphoid cells or 1-3 x 10^5 clonal antigen-specific T cells.

Cat. No.

Allele Peptide Antigen Disease/Organism
FA10001 DRB1*0101 PKYVKQNTLKLAT HA  Influenza
FA10002  DRB1*0101  PVSKMRMATPLLMQA CLIP  Endogenous Ag
FA10003  DRB1*0101  TSLYNLRRGTALA  EBNA1 EBV 
FA10004 DRB1*0101  PEQWMFQGAPPSQGT EBNA3A EBV 
FA10005  DRB1*0101  QEIYMQHTYPISA Tetanus Toxin Tetanus 
FA10006  DRB1*0101  LPLKMLNIPSINVH pp65 CMV 
FB10001  DRB1*0401  PKYVKQNTLKLAT HA Influenza
FB10002 DRB1*0401  PVSKMRMATPLLMQA CLIP Endogenous Ag
FB10012 DRB1*0401  GAGSLQPLALEGSLQKRG Proinsulin Diabetes 
FB10013 DRB1*0401  IAFTSEHSHFSLK  GAD65  Diabetes 
FB10014 DRB1*0401 NFIRMVISNPAAT GAD65  Diabetes 
FD10001 DRB1*1101 PKYVKQNTLKLAT HA Influenza
FD10002  DRB1*1101 PVSKMRMATPLLMQA CLIP Endogenous Ag
FD10020  DRB1*1101 EHPTFTSQYRIQGKL pp65 CMV
FH10002 DPB1*0401  PVSKMRMATPLLMQA CLIP Endogenous Ag
FH10035 DPB1*0401  SLLMWITQCFLPVF  NY-ESO Cancer 
FH10036  DPB1*0401   TQHFVQENYLEY  MAGE-A3  Cancer 
FH10037  DPB1*0401  KKYFAATQFEPLA Oxytocinase  Cancer

 

Test Size  Price (EUR) Price (USD)
50 tests 1.349* 1,549*
150 tests 2.599* 2,999*
500 tests 6.199* 7,099*
1000 tests 10.799* 12,349*

*for customized products an entry fee of 550EUR/ 650USD is added to evaluate each unique peptide's ability to bind to the requested MHC II allele using standardized peptide binding assays. 

Selected References for MHC II Dextramer®

  1. Massilamany C, Krishnan B, Reddy J. Major Histocompatibility Complex Class II Dextramers: New Tools for the Detection of antigen-Specific, CD4 T Cells in Basic and Clinical Research. Scand J Immunol. 2015;82(5):399-408.

  2. Krishnan B, et al. Versatility of using major histocompatibility complex class II dextramers for derivation and characterization of antigen-specific, autoreactive T cell hybridomas. J Immunol Methods. 2015;426:86-94.

  3. Massilamany C, et al. SJL mice infected with Acanthamoeba castellanii develop central nervous system autoimmunity through the generation of cross-reactive T cells for myelin antigens. PLoS One. Published 2014 May 30. 2014;9(5):e98506.

  4. Massilamany C, et al. In situ detection of autoreactive CD4 T cells in brain and heart using major histocompatibility complex class II dextramers. J Vis Exp. Published 2014 Aug 1. 2014;(90):e51679. 

  5. Massilamany C, et al. Direct staining with major histocompatibility complex class II dextramers permits detection of antigen-specific, autoreactive CD4 T cells in situ. PLoS One. Published 2014 Jan 27. 2014;9(1):e87519.

  6. Dolton G, Lissina A, Skowera A, et al. Comparison of peptide-major histocompatibility complex tetramers and dextramers for the identification of antigen-specific T cells. Clin Exp Immunol. 2014;177(1):47-63.

  7. Massilamany C, et al. Copper-zinc superoxide dismutase-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis induced with myelin oligodendrocyte glycoprotein 35-55. J Neuroimmunol. 2013;256(1-2):19-27.
     
  8. Gebregiworgis T, et al. Potential of urinary metabolites for diagnosing multiple sclerosis. ACS Chem Biol. 2013;8(4):684-690.

  9. Gangaplara A, et al. Coxsackievirus B3 infection leads to the generation of cardiac myosin heavy chain-α-reactive CD4 T cells in A/J mice. Clin Immunol. 2012;144(3):237-249.

  10. Massilamany C, et al. Detection of autoreactive CD4 T cells using major histocompatibility complex class II dextramers. BMC Immunol. Published 2011 Jul 18. 2011;12:40.

 

Our Technology

All our products are based on the following technologies:

Dextramer®Technology

dCODE® Technology

Klickmer™ Technology

Contact Us

Ordering
Email: ordering@immudex.com
Ordering: +45 29 13 42 24
Main: +45 31 10 91 91
Fax: +45 77 31 15 09
CVR/VAT no. 31348854

Technical Support
Email: customer@immudex.com

Want to Create Your Own Reagent?

Immudex' catalog includes more than 3500 different reagents, but if you need a reagent that is not listed in our catalog, or want to use the Dextramer®  Technology for research beyond T cells, you can choose one of our custom solutions.