MHC II Dextramer® – Identify Antigen-Specific CD4+ T Cells

Enhanced Sensitivity for Reliable Results

Typically expressed on the surface of antigen-presenting cells, MHC II molecules dock and primes CD4+ T cells.

Antigen-specific CD4+ T cells are critical for the immune response of many diseases, given their role in recruiting and coordinating other immune cells. Often challenging to detect and stratify with standard reagents, antigen-specific CD4+ T cells are revealed through the high-avidity and enhanced sensitivity of the Dextramer® technology.

With multiple MHC-peptide complexes and fluorophores on a flexible dextran backbone, MHC II Dextramer® reagents are designed for high-resolution characterization of CD4+ T-cell responses, even with low-abundance cells and in complex matrices like blood.

Dextramer® Technology

The backbone of precision immune monitoring

MHC II Dextramer® Flyer

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MHC Dextramer® Protocol

Download the staining protocol for single and multiple reagents

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Consult a list of the most common questions for MHC Dextramer®

MHC II Dextramer® detects DRB1*0101/EBV-specific T cells in a blood sample via flow cytometry (internal data).

MHC II Dextramer® detects DRB1*0101/EBV-specific T cells in a blood sample via flow cytometry (internal data).

A Tool For Meticulous And Precise Research

Push the boundaries of your flow cytometry assays with MHC II Dextramer® reagents. Designed for high avidity and exceptional signal-to-noise ratio, MHC II Dextramer® reagents enable you to detect antigen-specific CD4+ T cells with confidence.

  • Get the full picture of CD4+ T-cell responses, even the low-affinity ones.
  • Ensure consistent, reproducible, and comparable results.
  • Expand the limits of your research.
  • Secure flexibility in your experiments.
Discover Dextramer® Controls

Order MHC II Dextramer®

Use the webshop below to find and order your MHC II Dextramer® reagent online.

  1. If you can’t find a peptide specificity for your allele of choice in the webshop then you can specify and order your desired Dextramer® reagent with our custom tool below
  2. In case you don’t find your MHC allele of interest or other special requests then please contact us. Our Custom Solutions and Services has a very good track record of taking on custom projects and producing new alleles.
  3. Alternatively, contact us specifying:
  • Catalog number or desired MHC allele-peptide combination:
    - Consult our lists of available MHC alleles
  • Fluorophore (BV421, PE, APC, FITC, or none)
  • Test size (50,150, 500, 1000 tests). One test is 10 µl reagent and sufficient to stain 1–3 x 106 lymphoid cells or 1–3 x 105 clonal antigen-specific T cells.

Can’t find what you’re looking for?

Our webshop does not include all products. Contact us or email [email protected] and we will search our larger internal database of products for you. We also provide custom alleles and specificities.

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For custom MHC-peptide combinations, a one-time entry fee will be required to evaluate each unique peptide's ability to bind to the requested MHC allele using standardized peptide binding assays. If the refolding process is unsuccessful, only the entry fee will be charged as we will not be able to deliver a product to you. The entry fee for custom MHC II Dextramer® reagents is 605 EUR / 710 USD.

BV421 is equivalent to Brilliant Violet™ 421, which is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by BD Innovation.