All our products are based on the following technologies:
MHC II Dextramer®
Identify Antigen-specific CD4+ T Cells
Antigen-specific CD4+ T cells play an important role in the immune response of autoimmune disease, cancer, transplantation, infectious disease, and allergy.
MHC II Dextramer® reagents can identify antigen-specific CD4+ T-cell subsets by flow cytometry.
MHC II Dextramer reagents consist of a protein-stabilizing dextran backbone, an optimized number of MHC-peptide complexes, and fluorophores (PE, APC or FITC) or no fluorophore (None).
Immudex manufactures MHC II Dextramer reagents as listed in the table below. Customized reagents are easily made with MHC alleles from our catalog and with peptides that form stable complexes with the MHC molecule.
Detection of DRB1*0101/EBV-specific T cells in blood sample using flow cytometry.
How to Order MHC II Dextramer®
Please send an e-mail to ordering@immudex.com specifying:
- Cat. no or the MHC allele-peptide combination of the MHC II Dextramer reagent.
See our current list of MHC alleles - Fluorophore (PE, APC or FITC) or no Fluorophore (None)
- Test sizes (50, 150, 500, 1000 tests). One test is defined as 10 µl MHC Dextramer reagent, sufficient to stain up to 1-3 x 10^6 lymphoid cells or 1-3 x 10^5 clonal antigen-specific T cells.
|
Cat. No. |
Allele | Peptide | Antigen | Disease/Organism |
| FA10001 | DRB1*0101 | PKYVKQNTLKLAT | HA | Influenza |
| FA10002 | DRB1*0101 | PVSKMRMATPLLMQA | CLIP | Endogenous Ag |
| FA10003 | DRB1*0101 | TSLYNLRRGTALA | EBNA1 | EBV |
| FA10004 | DRB1*0101 | PEQWMFQGAPPSQGT | EBNA3A | EBV |
| FA10005 | DRB1*0101 | QEIYMQHTYPISA | Tetanus Toxin | Tetanus |
| FA10006 | DRB1*0101 | LPLKMLNIPSINVH | pp65 | CMV |
| FB10001 | DRB1*0401 | PKYVKQNTLKLAT | HA | Influenza |
| FB10002 | DRB1*0401 | PVSKMRMATPLLMQA | CLIP | Endogenous Ag |
| FB10012 | DRB1*0401 | GAGSLQPLALEGSLQKRG | Proinsulin | Diabetes |
| FB10013 | DRB1*0401 | IAFTSEHSHFSLK | GAD65 | Diabetes |
| FB10014 | DRB1*0401 | NFIRMVISNPAAT | GAD65 | Diabetes |
| FD10001 | DRB1*1101 | PKYVKQNTLKLAT | HA | Influenza |
| FD10002 | DRB1*1101 | PVSKMRMATPLLMQA | CLIP | Endogenous Ag |
| FD10020 | DRB1*1101 | EHPTFTSQYRIQGKL | pp65 | CMV |
| FH10002 | DPB1*0401 | PVSKMRMATPLLMQA | CLIP | Endogenous Ag |
| FH10035 | DPB1*0401 | SLLMWITQCFLPVF | NY-ESO | Cancer |
| FH10036 | DPB1*0401 | TQHFVQENYLEY | MAGE-A3 | Cancer |
| FH10037 | DPB1*0401 | KKYFAATQFEPLA | Oxytocinase | Cancer |
| Test Size | Price (EUR) | Price (USD) |
| 50 tests | 1.349* | 1,549* |
| 150 tests | 2.599* | 2,999* |
| 500 tests | 6.199* | 7,099* |
| 1000 tests | 10.799* | 12,349* |
*for customized products an entry fee of 550EUR/ 650USD is added to evaluate each unique peptide's ability to bind to the requested MHC II allele using standardized peptide binding assays.
Selected References for MHC II Dextramer®
- Massilamany C, Krishnan B, Reddy J. Major Histocompatibility Complex Class II Dextramers: New Tools for the Detection of antigen-Specific, CD4 T Cells in Basic and Clinical Research. Scand J Immunol. 2015;82(5):399-408.
- Krishnan B, et al. Versatility of using major histocompatibility complex class II dextramers for derivation and characterization of antigen-specific, autoreactive T cell hybridomas. J Immunol Methods. 2015;426:86-94.
- Massilamany C, et al. SJL mice infected with Acanthamoeba castellanii develop central nervous system autoimmunity through the generation of cross-reactive T cells for myelin antigens. PLoS One. Published 2014 May 30. 2014;9(5):e98506.
- Massilamany C, et al. In situ detection of autoreactive CD4 T cells in brain and heart using major histocompatibility complex class II dextramers. J Vis Exp. Published 2014 Aug 1. 2014;(90):e51679.
- Massilamany C, et al. Direct staining with major histocompatibility complex class II dextramers permits detection of antigen-specific, autoreactive CD4 T cells in situ. PLoS One. Published 2014 Jan 27. 2014;9(1):e87519.
- Dolton G, Lissina A, Skowera A, et al. Comparison of peptide-major histocompatibility complex tetramers and dextramers for the identification of antigen-specific T cells. Clin Exp Immunol. 2014;177(1):47-63.
- Massilamany C, et al. Copper-zinc superoxide dismutase-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis induced with myelin oligodendrocyte glycoprotein 35-55. J Neuroimmunol. 2013;256(1-2):19-27.
- Gebregiworgis T, et al. Potential of urinary metabolites for diagnosing multiple sclerosis. ACS Chem Biol. 2013;8(4):684-690.
- Gangaplara A, et al. Coxsackievirus B3 infection leads to the generation of cardiac myosin heavy chain-α-reactive CD4 T cells in A/J mice. Clin Immunol. 2012;144(3):237-249.
- Massilamany C, et al. Detection of autoreactive CD4 T cells using major histocompatibility complex class II dextramers. BMC Immunol. Published 2011 Jul 18. 2011;12:40.
Contact Us
Ordering
Email: ordering@immudex.com
Ordering: +45 29 13 42 24
Main: +45 31 10 91 91
Fax: +45 77 31 15 09
CVR/VAT no. 31348854
Technical Support
Email: customer@immudex.com
Want to Create Your Own Reagent?
Immudex' catalog includes more than 3500 different reagents, but if you need a reagent that is not listed in our catalog, or want to use the Dextramer® Technology for research beyond T cells, you can choose one of our custom solutions.
