For T-cell Research and Beyond
Klickmer™ is a Dextramer® backbone, carrying multiple acceptor sites, enabling binding of a high number of biotinylated molecules to generate high avidity multimers, displaying the molecule of choice. Klickmer reagents can be fluorescently labelled (PE/FITC/APC) or unlabelled.
Klickmer is also available in a DNA barcode labelled version, dCODE® Klickmer, for multi-omics analysis. Find out more about dCODE® Klickmer
Any biotinylated molecule can be attached to a Klickmer. This multipurpose tool serves a range of applications and can help address diverse needs in research and development.
- Highly sensitive detection of antigen-specific T cells and B cells
- Simulation of antigen presentation
- Identification of ligands for low-affinity interactions
- Isolation of specific cell populations
- Amplification of the signal on your cell populations
Additionally, using MHC-Klickmer reagent, it is possible to create a Dextramer reagent simultaneously displaying peptide-loaded MHC complexes and other molecules of choice (Ref. 10). For more information about MHC-Klickmer reagents, please contact us.
How to Order Klickmer™
For Klickmer and MHC Klickmer, define your choice of fluorochrome and volume and send an email to firstname.lastname@example.org
To order dCODE® Klickmer, please send an email to email@example.com
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- Dolton G, et al. Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells. Front Immunol. Published 2018 Jun 29. 2018;9:1378.
- Luque S, et al. A multicolour HLA-specific B-cell FluoroSpot assay to functionally track circulating HLA-specific memory B cells. J Immunol Methods. 2018;462:23-33.
- Bentzen AK, et al. T cell receptor fingerprinting enables in-depth characterization of the interactions governing recognition of peptide-MHC complexes [published online ahead of print, 2018 Nov 19]. Nat Biotechnol. 2018;10.1038/nbt.4303.
- Chancellor A, et al. CD1b-restricted GEM T cell responses are modulated by Mycobacterium tuberculosis mycolic acid meromycolate chains. Proc Natl Acad Sci U S A. 2017;114(51):E10956-E10964.
- Massilamany C, et al. Major Histocompatibility Complex Class II Dextramers: New Tools for the Detection of antigen-Specific, CD4 T Cells in Basic and Clinical Research. Scand J Immunol. 2015;82(5):399-408.
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- Kasmar AG, et al. Cutting Edge: CD1a tetramers and dextramers identify human lipopeptide-specific T cells ex vivo. J Immunol. 2013;191(9):4499-4503.
- Yang GB, et al. Immunization with recombinant macaque major histocompatibility complex class I and II and human immunodeficiency virus gp140 inhibits simian-human immunodeficiency virus infection in macaques. J Gen Virol. 2012;93(Pt 7):1506-1518.
- Mörner A, et al. Immunization with recombinant HLA classes I and II, HIV-1 gp140, and SIV p27 elicits protection against heterologous SHIV infection in rhesus macaques. J Virol. 2011;85(13):6442-6452.
- Wang Y, et al. P19-20. Allogeneic stimulation of the anti-viral APOBEC3G in human CD4+ T cells and prevention of SHIV infectivity in macaques immunized with HLA antigens. Retrovirology. Published 2009 Oct 22. 2009;6(Suppl 3):P340.