Klickmer™

For T-cell Research and Beyond

Klickmer is a Dextramer® backbone, carrying multiple acceptor sites, enabling binding of a high number of biotinylated molecules to generate high avidity multimers, displaying the molecule of choice. Klickmer reagents can be fluorescently labelled (PE/FITC/APC) or unlabelled. 

Klickmer is also available in a DNA barcode labelled version, dCODE® Klickmer, for multi-omics analysis. Find out more about dCODE® Klickmer

Klickmer Applications

Any biotinylated molecule can be attached to a Klickmer. This multipurpose tool serves a range of applications and can help address diverse needs in research and development.

  • Highly sensitive detection of antigen-specific T cells and B cells
  • Simulation of antigen presentation
  • Identification of ligands for low-affinity interactions
  • Isolation of specific cell populations
  • Amplification of the signal on your cell populations

Additionally, using MHC-Klickmer reagent, it is possible to create a Dextramer reagent simultaneously displaying peptide-loaded MHC complexes and other molecules of choice (Ref. 10). For more information about MHC-Klickmer reagents, please contact us.

How to Order Klickmer™

For Klickmer and MHC Klickmer, define your choice of fluorochrome and volume and send an email to ordering@immudex.com

Fluorochrome Cat. Number Volume Price (EUR) Price (USD)
Klickmer-PE/APC/FITC/none DX01-PE/APC/FITC/none 200 µl 2.499 2,849
    1000 µl 5.199 5,999
    2000 µl 8.999 9,999

To order dCODE® Klickmer, please send an email to customer@immudex.com

References

  1. Johnston RJ, et al. VISTA is an acidic pH-selective ligand for PSGL-1. Nature. 2019;574(7779):565-570. 

  2. Dolton G, et al. Optimized Peptide-MHC Multimer Protocols for Detection and Isolation of Autoimmune T-Cells. Front Immunol. Published 2018 Jun 29. 2018;9:1378.

  3. Luque S, et al. A multicolour HLA-specific B-cell FluoroSpot assay to functionally track circulating HLA-specific memory B cells. J Immunol Methods. 2018;462:23-33.

  4. Bentzen AK, et al. T cell receptor fingerprinting enables in-depth characterization of the interactions governing recognition of peptide-MHC complexes [published online ahead of print, 2018 Nov 19]. Nat Biotechnol. 2018;10.1038/nbt.4303.

  5. Chancellor A, et al. CD1b-restricted GEM T cell responses are modulated by Mycobacterium tuberculosis mycolic acid meromycolate chains. Proc Natl Acad Sci U S A. 2017;114(51):E10956-E10964.

  6. Massilamany C, et al. Major Histocompatibility Complex Class II Dextramers: New Tools for the Detection of antigen-Specific, CD4 T Cells in Basic and Clinical Research. Scand J Immunol. 2015;82(5):399-408.

  7. Neller MA, et al. Naive CD8⁺ T-cell precursors display structured TCR repertoires and composite antigen-driven selection dynamics. Immunol Cell Biol. 2015;93(7):625-633. 

  8. Lolli F, et al. Increased CD8+ T cell responses to apoptotic T cell-associated antigens in multiple sclerosis. J Neuroinflammation. Published 2013 Jul 27. 2013;10:94.

  9. Kasmar AG, et al. Cutting Edge: CD1a tetramers and dextramers identify human lipopeptide-specific T cells ex vivo. J Immunol. 2013;191(9):4499-4503.

  10. Yang GB, et al. Immunization with recombinant macaque major histocompatibility complex class I and II and human immunodeficiency virus gp140 inhibits simian-human immunodeficiency virus infection in macaques. J Gen Virol. 2012;93(Pt 7):1506-1518.

  11. Mörner A, et al. Immunization with recombinant HLA classes I and II, HIV-1 gp140, and SIV p27 elicits protection against heterologous SHIV infection in rhesus macaques. J Virol. 2011;85(13):6442-6452.

  12. Wang Y, et al. P19-20. Allogeneic stimulation of the anti-viral APOBEC3G in human CD4+ T cells and prevention of SHIV infectivity in macaques immunized with HLA antigens. Retrovirology. Published 2009 Oct 22. 2009;6(Suppl 3):P340.

Our Technology

All our products are based on the following technologies:

Dextramer®Technology

dCODE® Technology

Klickmer™ Technology

Contact Us

Ordering
Email: ordering@immudex.com
Ordering: +45 29 13 42 24
Main: +45 31 10 91 91
Fax: +45 77 31 15 09
CVR/VAT no. 31348854

Technical Support
Email: customer@immudex.com