Dextramer® Controls – Best Practices in Research Call for the Best Controls in Experiments

Controls eliminate confounding factors, distinguish signal from background, and help troubleshoot protocols

Order Dextramer® Controls

Couple your Dextramer® reagents with the right positive and negative controls. Immudex offers different options to design Dextramer® Positive and Negative Controls with fluorophore labelling (PE, APC, and FITC) for your flow cytometry and immunohistochemistry experiments. See the allele and antigen combinations we propose for Positive and Negative controls.

To order, send an email to [email protected] specifying:

• Catalog number or desired MHC allele-peptide combination
• Fluorophore (PE, APC, FITC or none)
• Test size (50,150, 500, 1000 tests). One test is 10 µl reagent and sufficient to stain 1–3 x 10⁶ lymphid cells or 1–3 x 10⁵ clonal antigen-specific T cells

*Custom products incur an additional entry fee of 550EUR/650USD to evaluate peptide binding to the requested MHC II allele using standardized binding assays.

Dextramer® Controls

Depending on your experimental setup, you can order Dextramer^® Negative Controls carrying:

• a non-sense peptide sequences for Human MHC I allele
• a peptide sequence unrelated to your experiment
• a CLIP peptide for Human MHC II allele

Depending on your experimental setup, you can order Dextramer^® Positive Controls that:

• recognize epitopes derived from widespread human viruses (CMV, EBV, and Flu)
• carry three viral epitopes from CMV, EBV and Flu and are then pooled

*H2-Kb/SIYRYYGL (SIY) can be used as a positive and negative control depending on the mouse model. For example, in OVA mouse models, it can serve as a negative control reagent, while H-2KB/SIINFEKL is the positive control reagent [1,2].

*H2-Kb/SIYRYYGL (SIY) can be used as a positive and negative control depending on the mouse model. For example, this peptide is expressed in certain mouse models for tumors and recognized by CD8+ T cells expressing 2C TCR. Thus, it can be used as a positive control [4,5].

^{For custom MHC-peptide combinations, a one-time entry fee will be required to evaluate each unique peptide's ability to bind to the requested MHC allele using standardized peptide binding assays. If the refolding process is unsuccessful, only the entry fee will be charged as we will not be able to deliver a product to you. The entry fee for custom MHC I Dextramer® reagents is 375 EUR / 435 USD and the entry fee for custom MHC II Dextramer® reagents is 590 EUR / 695 USD.}

Dextramer® Custom Controls

Your experimental setup may require an MHC allele that is not listed in our catalog or different specificities. Our Custom Solutions and Services Team can ensure you have the right controls for your research. Just contact us.