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Dextramer® Controls – Best Practices in Research Call for the Best Controls in Experiments

Controls eliminate confounding factors, distinguish signal from background, and help troubleshoot protocols

Controls Are the Core of Every Experiment

Positive and negative controls are often an afterthought in the design of experiments. And yet, without them, results leave room for ambiguity. Only properly controlled experiments can concretely discriminate hypotheses.

Controls also help resolve technical issues in experiments:

MHC I Dextramer® Negative Control staining allows to distinguish a MART-1-specific CD8+ T-cell population from background staining using flow cytometry.

Order Dextramer® Controls

Couple your Dextramer® reagents with the right positive and negative controls. Immudex offers different options to design Dextramer® Positive and Negative Controls with fluorophore labelling (PE, APC, and FITC) for your flow cytometry and immunohistochemistry experiments. See the allele and antigen combinations we propose for Positive and Negative controls.

To order, send an email to ordering@immudex.com specifying:

  • Catalog number or desired MHC allele-peptide combination
  • Fluorophore (PE, APC, FITC or none)
  • Test size (50,150, 500, 1000 tests). One test is 10 µl reagent and sufficient to stain 1–3 x 106 lymphid cells or 1–3 x 105 clonal antigen-specific T cells

Dextramer® Positive and Negative Controls

Test size

Price (€)*

Price ($)*

Human and Mouse MHC I

 

50 tests

 1,265 1.490

150 tests

 2,360 2.645

500 tests

 5,675 6.410

1000 tests

 10,785 12.185

Human MHC II

50 tests

 1,435 1.645

150 tests

 2,760 3.185

500 tests

 6,575 7.535

1000 tests

 12,495 14.315

*Custom products incur an additional entry fee of 550EUR/650USD to evaluate peptide binding to the requested MHC II allele using standardized binding assays.

Dextramer® Negative Controls

Depending on your experimental setup, you can order Dextramer® Negative Controls carrying:

  • a non-sense peptide sequences for Human MHC I allele
  • a peptide sequence unrelated to your experiment
  • a CLIP peptide for Human MHC II allele

Type 

Allele 

Antigen 

Epitope 

Cat. No. 

Human MHC I 

HLA-A*0101 

Neg. Control 

STEGGGLAY   

WA03579 

Human MHC I 

HLA-A*0201 

Neg. Control 

ALIAPVHAV 

WB02666 

Human MHC I 

HLA-A*0301  

Neg. Control 

GLFGAGAFK 

WC03191 

Human MHC I 

HLA-B*0801 

Neg. Control 

AAKGRGAAL 

WI03233 

Mouse MHC I 

H-2 Kb 

SIY* 

SIYRYYGL 

JD02164 

Mouse MHC I 

H-2 Kd 

Neg. Control 

AYAGSAGSI 

JE03929 

Human MHC II 

HLA-DRB1*0101 

CLIP (Endogenous Ag) 

PVSKMRMATPLLMQA 

FA10002 

Human MHC II 

HLA-DRB1*0301 

CLIP (Endogenous Ag) 

PVSKMRMATPLLMQA 

FF10002 

Human MHC II 

HLA-DRB1*0401 

CLIP (Endogenous Ag) 

PVSKMRMATPLLMQA 

FB10002 

Human MHC II 

HLA-DRB1*0701 

CLIP (Endogenous Ag) 

PVSKMRMATPLLMQA 

FE10002 

Human MHC II 

HLA-DRB1*1101 

CLIP (Endogenous Ag) 

PVSKMRMATPLLMQA 

FD10002 

Human MHC II 

HLA-DRB1*1301 

CLIP (Endogenous Ag) 

PVSKMRMATPLLMQA 

FK10002 

Human MHC II 

HLA-DRB1*1501 

CLIP (Endogenous Ag) 

PVSKMRMATPLLMQA 

FC10002 

Human MHC II 

HLA-DQ2.5 

CLIP2 (Endogenous Ag) 

PVSKMRMATPLLMQA 

FI10188 

*H2-Kb/SIYRYYGL (SIY) can be used as a positive and negative control depending on the mouse model. For example, in OVA mouse models, it can serve as a negative control reagent, while H-2KB/SIINFEKL is the positive control reagent [1,2].

Dextramer® Positive Controls

Depending on your experimental setup, you can order Dextramer® Positive Controls that:

  • recognize epitopes derived from widespread human viruses (CMV, EBV, and Flu)
  • carry three viral epitopes from CMV, EBV and Flu and are then pooled

Type

Allele

Peptide

Antigen

Cat. No.

Human MHC I

HLA-A*0201

NLVPMVATV

CMV

WB02132

Human MHC I

HLA-A*0201

GLCTLVAML

EBV

WB02130

Human MHC I

HLA-A*0201

GILGFVFTL

Flu

WB02161

Mouse MHC I

H-2 Kb

SIYRYYGL

SIY*

JD02164

*H2-Kb/SIYRYYGL (SIY) can be used as a positive and negative control depending on the mouse model. For example, this peptide is expressed in certain mouse models for tumors and recognized by CD8+ T cells expressing 2C TCR. Thus, it can be used as a positive control [4,5].

Dextramer® Custom Controls

Your experimental setup may require an MHC allele that is not listed in our catalog or different specificities. Our Custom Solutions and Services Team can ensure you have the right controls for your research. Just contact us.

Selected References