Dextramer® Controls – Best Practices in Research Call for the Best Controls in Experiments
Controls eliminate confounding factors, distinguish signal from background, and help troubleshoot protocols
Controls Are the Core of Every Experiment
Positive and negative controls are often an afterthought in the design of experiments. And yet, without them, results leave room for ambiguity. Only properly controlled experiments can concretely discriminate hypotheses.
Controls also help resolve technical issues in experiments:
MHC I Dextramer® Negative Control staining allows to distinguish a MART-1-specific CD8+ T-cell population from background staining using flow cytometry.
Order Dextramer® Controls
Couple your Dextramer® reagents with the right positive and negative controls. Immudex offers different options to design Dextramer® Positive and Negative Controls with fluorophore labelling (PE, APC, and FITC) for your flow cytometry and immunohistochemistry experiments. See the allele and antigen combinations we propose for Positive and Negative controls.
Catalog number or desired MHC allele-peptide combination
Fluorophore (PE, APC, FITC or none)
Test size (50,150, 500, 1000 tests). One test is 10 µl reagent and sufficient to stain 1–3 x 106 lymphid cells or 1–3 x 105 clonal antigen-specific T cells
*Custom products incur an additional entry fee of 550EUR/650USD to evaluate peptide binding to the requested MHC II allele using standardized binding assays.
Depending on your experimental setup, you can order Dextramer® Negative Controls carrying:
a non-sense peptide sequences for Human MHC I allele
a peptide sequence unrelated to your experiment
a CLIP peptide for Human MHC II allele
Depending on your experimental setup, you can order Dextramer® Positive Controls that:
recognize epitopes derived from widespread human viruses (CMV, EBV, and Flu)
carry three viral epitopes from CMV, EBV and Flu and are then pooled
*H2-Kb/SIYRYYGL (SIY) can be used as a positive and negative control depending on the mouse model. For example, in OVA mouse models, it can serve as a negative control reagent, while H-2KB/SIINFEKL is the positive control reagent [1,2].
*H2-Kb/SIYRYYGL (SIY) can be used as a positive and negative control depending on the mouse model. For example, this peptide is expressed in certain mouse models for tumors and recognized by CD8+ T cells expressing 2C TCR. Thus, it can be used as a positive control [4,5].
Can’t find what you’re looking for?
Our webshop is currently undergoing maintenance. Contact us or email [email protected] and we will search our larger internal database of products for you. We also provide custom alleles and specificities.
For custom MHC-peptide combinations, a one-time entry fee will be required to evaluate each unique peptide's ability to bind to the requested MHC allele using standardized peptide binding assays. If the refolding process is unsuccessful, only the entry fee will be charged as we will not be able to deliver a product to you. The entry fee for custom MHC I Dextramer® reagents is 375 EUR / 435 USD and the entry fee for custom MHC II Dextramer® reagents is 590 EUR / 695 USD.
Dextramer® Custom Controls
Your experimental setup may require an MHC allele that is not listed in our catalog or different specificities. Our Custom Solutions and Services Team can ensure you have the right controls for your research. Just contact us.