Reliable Identification of SARS-CoV-2-Specific T-Cell Responses

 

Explore T-Cell Immunity in COVID-19

A deeper understanding of virus-specific T-cell responses to COVID-19 is needed to evaluate vaccine candidates, explore long-term immunity, predict patients at risk, and even prepare for the next pandemic.

Dextramer® technology can help you monitor and characterize SARS-CoV-2- specific CD4+ and CD8+ T cells in blood, without missing rare or low-affinity cells, in an easy and reproducible way.​

Learn More about T Cells and COVID-19

Frequencies of SARS-CoV-2-specific CD8+ T cells detected in PBMCs at different time points from a vaccinated convalescent donor with a SARS-CoV-2 MHC I Panel of Non-Spike Epitopes.

SARS-CoV-2 Dextramer® Panels

We provide SARS-CoV-2 Dextramer® reagents in panels targeting Spike or Non-Spike epitopes, each with a selection of validated T-cell epitopes.

Alternatively, select your own custom panel based on our selection of SARS-CoV-2 Dextramer® reagents, or order individual Dextramer® reagents.

The immunodominant SARS-CoV-2 specific T-cell epitopes listed below are known to be present in large populations, based on publications and in-house knowledge, enabling researchers to monitor T-cell immunity across COVID-19 variants.

SARS-CoV-2 MHC I Panel of Spike Epitopes

Allele

Peptide

Antigen

Delta

Omicron BA 2.12.1

Omicron BA.4

Omicron BA. 5

A*0101

LTDEMIAQY

S

 

 

 

 

A*0101

WTAGAAAYY

S

 

 

 

 

A*0201

YLQPRTFLL

S

 

 

 

 

A*0201

FIAGLIAIV

S

 

 

 

 

A*0301

KCYGVSPTK

S

 

 

 

 

A*0301

GVYFASTEK

S

 

GVYFASIEK

 

 

A*1101

RLFRKSNLK

S

 

 

 

 

A*1101

KCYGVSPTK

S

 

 

 

 

A*1101

GVYFASTEK

S

 

GVYFASIEK

 

 

A*2402

QYIKWPWYI

S

 

 

 

 

A*2402

NYNYLYRLF

S

NYNYRYRLF

 

NYNYRYRLF

NYNYRYRLF

B*0702

SPRRARSVA

S

SRRRARSVA

SHRRARSVA

SHRRARSVA

SHRRARSVA

B*0702

APHGVVFL

S

 

 

 

 

B*3501

QPTESIVRF

S

 

 

 

 

B*3501

LPFNDGVYF

S

 

 

 

 

B*3501

IPFAMQMAY

S

 

 

 

 

Spike-specific CD8+ T-cell epitopes in the SARS-CoV-2 Dextramer® Panels, and the conservation of these epitopes across the SARS-CoV-2 Delta (B.1.617.2) and Omicron (BA 2.12.1, BA. 4, BA. 5) variants. All epitopes are conserved in the reference strain (NC_045512). Mutations in the epitope are shown in red. Learn more in this Application Note.

SARS-CoV-2 MHC I Panel of Non-Spike Epitopes

Allele

Peptide

Antigen

Delta

Omicron

Omicron BA.4

Omicron BA. 5

A*0201

LLLDRLNQL

N

 

 

 

 

A*0301

KTFPPTEPK

N

 

 

 

 

A*1101

KTFPPTEPK

N

 

 

 

 

B*0702

SPRWYFYYL

N

 

 

 

 

A*0101

FTSDYYQLY

ORF3a

 

 

 

 

A*0201

LLYDANYFL

ORF3a

 

 

 

 

A*2402

VYFLQSINF

ORF3a

 

 

 

 

A*0101

TTDPSFLGRY

ORF1ab

TTDLSFLGRY

 

 

 

A*0201

ALWEIQQVV

ORF1ab

 

 

 

 

A*0301

VVYRGTTTYK

ORF1ab

 

 

 

 

A*1101

ASMPTTIAK

ORF1ab

 

 

 

 

A*2402

VYIGDPAQL

ORF1ab

 

 

 

 

B*0702

IPRRNVATL

ORF1ab

 

 

 

 

Non-Spike-specific CD8+ T-cell epitopes in the SARS-CoV-2 Dextramer® Panels, and the conservation of these epitopes across the SARS-CoV-2 Delta (B.1.617.2), and Omicron (BA 2.12.1, BA. 4, BA. 5) variants. Green = no mutations in epitope, Red = mutations in epitope. All epitopes are conserved in the reference strain (NC_045512). Mutations in the epitope are shown in red. Learn more in this Application Note.

SARS-CoV-2 Dextramer® Reagents

All SARS-CoV-2 Dextramer® reagents are available for detection of antigen-specific T cells by in-situ staining, flow cytometry, NGS or single-cell multi-omics. All specificities listed below are also available as MHC Monomers.

MHC I or MHC II Dextramer® - for identification of antigen-specific T cells by flow cytometry

dCODE Dextramer® (HiT) - for bulk analysis of antigen-specific T cells by PCR and NGS

dCODE Dextramer® (10x) -  for single-cell multi-omic analysis of antigen-specific T cells to combine information on TCR recognition, TCR sequencing, and gene expression using 10x Chromium Single Cell analysis.

dCODE Dextramer® (RiO) - for single-cell multi-omic analysis of antigen-specific T cells to combine information on TCR recognition, TCR sequencing, and gene expression using BD RhapsodyTM Single-Cell Analysis.

MHC I or MHC II Monomers – for flexible investigation of antigen-specific T cells

                                          MHC Dextramer® Reagents

                                              dCODE Dextramer® Reagents

SARS-CoV-2 MHC I Reagents

Catalogue Number

Allele

Peptide

Antigen

WB05946

A*0201

SLVKPSFYV

E

WB05950

A*0201

KLLEQWNLV

M

WH05882

B*0702

LPKEITVAT

M

WD05859

A*1101

LSYFIASFR

M

WB03848

A*0201

TLACFVLAAV

M

WH05758

B*0702

APSASAFFGM

N

WD05981

A*1101

ATEGALNTPK

N

WC05981

A*0301

ATEGALNTPK

N

WB05751

A*0201

GMSRIGMEV

N

WB05848

A*0201

ILLNKHIDA

N

WH05888

B*0702

KPRQKRTAT

N

WC05754

A*0301

KTFPPTEPK

N

WD05754

A*1101

KTFPPTEPK

N

WB05977

A*0201

LLLDRLNQL

N

WL05761

B*4403

MEVTPSGTW

N

WJ05988

B*2705

QRNAPRITF

N

JD05842

B*0702

SPRWYFYYL

N

WK05759

B*3501

TPSGTWLTY

N

WB06025

A*0201

ALWEIQQVV

ORF1ab

WD06030

A*1101

ASMPTTIAK

ORF1ab

WD05983

A*1101

ATSRTLSYYK

ORF1ab

WA06028

A*0101

DTDFVNEFY

ORF1ab

WB06034

A*0201

FLLPSLATV

ORF1ab

WA06081

A*0101

GTDLEGNFY

ORF1ab

WA06115

A*0101

HTTDPSFLGRY

ORF1ab

WB05974

A*0201

ILFTRFFYV

ORF1ab

WH06032

B*0702

IPRRNVATL

ORF1ab

WB06036

A*0201

KLWAQCVQL

ORF1ab

WC06082

A*0301

KTIQPRVEK

ORF1ab

WA06029

A*0101

PTDNYITTY

ORF1ab

WB06043

A*0201

RTIKVFTTV

ORF1ab

WD06083

A*1101

SAFAMMFVK

ORF1ab

WL05990

B*4402

SEFSSLPSY

ORF1ab

WD05982

A*1101

STFNVPMEK

ORF1ab

WA06115

A*0101

TTDPSFLGRY

ORF1ab

WC05978

A*0301

VVYRGTTTYK

ORF1ab

WF05984

A*2402

VYIGDPAQL

ORF1ab

WB06039

A*0201

YLFDESGEFKL

ORF1ab

WC06082

A*0301

KTIQPRVEK

ORF1ab

WA06027

A*0101

CTDDNALAYY

ORF1ab

WB05948

A*0201

ALSKGVHFV

ORF3a

WA05973

A*0101

FTSDYYQLY

ORF3a

WB05939

A*0201

LLYDANYFL

ORF3a

WF06031

A*2402

VYFLQSINF

ORF3a

WB05949

A*0201

HLVDFQVTI

ORF6

WH06103

B*0702

APHGVVFL

S

WB05825

A*0201

FIAGLIAIV

S

WB05975

A*0201

FLHVTYVPA

S

WC05853

A*0301

GVYFASTEK

S

WD05853

A*1101

GVYFASTEK

S

WD05861

A*1101

GVYYHKNNK

S

WK06107

B*3501

IPFAMQMAY

S

WD05979

A*1101

KCYGVSPTK

S

WC05979

A*0301

KCYGVSPTK

S

WA05846

A*0101

LTDEMIAQY

S

WI05885

B*0801

MIAQYTSAL

S

WH05885

B*0702

MIAQYTSAL

S

WC05857

A*0301

MTSCCSCLK

S

WD05857

A*1101

MTSCCSCLK

S

WB05850

A*0201

NLNESLIDL

S

WF06097

A*2402

NYNYLYRLF

S

WC05856

A*0301

QIYKTPPIK

S

WK06104

B*3501

QPTESIVRF

S

WF05952

A*2402

QYIKWPWYI

S

WD05854

A*1101

RLFRKSNLK

S

WC05854

A*0301

RLFRKSNLK

S

WB05976

A*0201

RLITGRLQSL

S

WB05750

A*0201

RLNEVAKNL

S

WB05914

A*0201

RLQSLQTYV

S

WH05879

B*0702

SPRRARSVA

S

WB05947

A*0201

TLDSKTQSL

S

WD05855

A*1101

TLKSFTVEK

S

WC05855

A*0301

TLKSFTVEK

S

WB05823

A*0201

VLNDILSRL

S

WB05849

A*0201

VVFLHVTYV

S

WA05847

A*0101

WTAGAAAYY

S

WB05824

A*0201

YLQPRTFLL

S

WK06106

B*3501

LPFNDGVYF

S

SARS-CoV-2 MHC II Reagents

Catalogue Number

Allele

Peptide

Antigen

FA10172

DRB1*0101

FYVYSRVKNLNSSRV

E

FB10172

DRB1*0401

FYVYSRVKNLNSSRV

E

FE10172

DRB1*0701

FYVYSRVKNLNSSRV

E

FA10170

DRB1*0101

LLLLDRLNQLESKMS

N

FA10171

DRB1*0101

LEASFNYLKSPNFSK

ORF1ab

FE10173

DRB1*0101

ESPFVMMSAPPAQYE

ORF1ab

FA10174

DRB1*0101

EEIAIILASFSASTS

ORF1ab

FA10175

DRB1*0101

EAFEKMVSLLSVLLS

ORF1ab

FB10171

DRB1*0401

LEASFNYLKSPNFSK

ORF1ab

FB10174

DRB1*0401

EEIAIILASFSASTS

ORF1ab

FB10175

DRB1*0401

EAFEKMVSLLSVLLS

ORF1ab

FE10171

DRB1*0701

LEASFNYLKSPNFSK

ORF1ab

FE10174

DRB1*0701

EEIAIILASFSASTS

ORF1ab

FE10175

DRB1*0701

EAFEKMVSLLSVLLS

ORF1ab

FA10157

DRB1*0101

QLIRAAEIRASANLAATK

S

FA10160

DRB1*0101

GAALQIPFAMQMAYRF

S

FA10161

DRB1*0101

MAYRFNGIGVTQNVLY

S

FA10162

DRB1*0101

QALNTLVKQLSSNFGAI

S

FA10164

DRB1*0101

SASFSTFKCYGVSPTKL

S

FA10165

DRB1*0101

KSFTVEKGIYQTSNFRVQ

S

FA10167

DRB1*0101

HWFVTQRNFYEPQII

S

FA10168

DRB1*0101

QSIIAYTMSLGAENSVAY

S

FA10169

DRB1*0101

DSLSSTASALGKLQDVV

S

FB10157

DRB1*0401

QLIRAAEIRASANLAATK

S

FB10161

DRB1*0401

MAYRFNGIGVTQNVLY

S

FB10162

DRB1*0401

QALNTLVKQLSSNFGAI

S

FB10165

DRB1*0401

KSFTVEKGIYQTSNFRVQ

S

FB10167

DRB1*0401

HWFVTQRNFYEPQII

S

FB10168

DRB1*0401

QSIIAYTMSLGAENSVAY

S

FE10157

DRB1*0701

QLIRAAEIRASANLAATK

S

FE10160

DRB1*0701

GAALQIPFAMQMAYRF

S

FE10161

DRB1*0701

MAYRFNGIGVTQNVLY

S

FE10164

DRB1*0701

SASFSTFKCYGVSPTKL

S

FE10165

DRB1*0701

KSFTVEKGIYQTSNFRVQ

S

FE10166

DRB1*0701

VKQIYKTPPIKDFGGFNF

S

FE10167

DRB1*0701

HWFVTQRNFYEPQII

S

FE10168

DRB1*0701

QSIIAYTMSLGAENSVAY

S

Order your SARS-CoV-2 Panels

Please contact our application specialist if you would like to discuss your research.

Please include information on:

  • Your preferred epitope(s) and alleles for MHC Dextramer®, dCODE Dextramer®, or MHC Monomer
  • Your preferred reagent format: MHC Dextramer® or dCODE Dextramer®
  • Your preferred dye (for MHC Dextramer®): PE, APC, FITC, or None
  • Test size:
    o MHC Dextramer®, select between 50 or 150 tests
    o dCODE Dextramer®, select between 25, 50, or 150 tests
    o MHC Monomers select between 20 µg (~50 tests), 100 µg (~250 tests), or 200 µg (~500 tests)

Published Human COVID-19 T-Cell Epitopes

Download the list of epitopes in COVID-19 infected or vaccinated individuals

Published Mouse COVID-19 T-Cell Epitopes

Download the list of epitopes in COVID-19 infected genetically modified mice.

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