Practical advice to set up and troubleshoot your flow cytometry experiment for immune monitoring.
The Fluorescence Signal is Too High
A fluorescence signal that is too high can cause signal spreading into adjacent fluorescence channels, decreasing their resolution. Many flow cytometers – especially of older ones – have non-linear responses in the higher range. A signal that lies in the higher range influences the compensation of the spectral spillover between the affected channel and adjacent channels.
The resulting over- or under-compensation can distort data. Make sure to titrate the reagents for optimal separation, keeping the signal within the linear part of the scale and minimizing spreading into adjacent channels.
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MHC Dextramer® Reagents
Sensitively quantify target immune cells by flow cytometry with a strong signal-to-noise ratio.