The Fluorescence Signal is Too High

A fluorescence signal that is too high can cause signal spreading into adjacent fluorescence channels, decreasing their resolution. Many flow cytometers – especially of older ones – have non-linear responses in the higher range. A signal that lies in the higher range influences the compensation of the spectral spillover between the affected channel and adjacent channels.

The resulting over- or under-compensation can distort data. Make sure to titrate the reagents for optimal separation, keeping the signal within the linear part of the scale and minimizing spreading into adjacent channels.

Further Help with Troubleshooting Flow Cytometry

Troubleshooting Flow

Practical advice to set up and troubleshoot your flow cytometry experiment for immune monitoring.

Tips and Tricks

Find a collection of various Tips and Tricks to optimize your experiment.

Technical Support

Contact our technical experts for further assistance with troubleshooting.

MHC Dextramer® Reagents

Sensitively quantify target immune cells by flow cytometry with a strong signal-to-noise ratio.