Practical advice to set up and troubleshoot your flow cytometry experiment for immune monitoring.
The Scatter Profile is Unusual
Incorrect instrument settings can lead to an unusual scatter profile. Check your settings and adjust them if necessary. A compromised sample can also cause distortion of the scatter plot. For example, if blood cells are handled inappropriately, have been starved or otherwise stressed, some may undergo degranulation, activation or even cell death, which impact the data collected by the cytometer. Thus, prepare and handle your sample with care during staining.
Further Help with Troubleshooting Flow Cytometry
Tips and Tricks
Find a collection of various Tips and Tricks to optimize your experiment.
Contact our technical experts for further assistance with troubleshooting.
MHC Dextramer® Reagents
Sensitively quantify target immune cells by flow cytometry with a strong signal-to-noise ratio.