T-cell ELISpot Proficiency Panel

How Well Do You Monitor T-cell Subsets Using

the ELISpot Assay?

Laboratories around the world are invited to participate in Immudex' annual T-cell ELISpot Proficiency Panel.

In the panel, participants get a chance to assess their proficiency in detecting the number of IFN-γ secreting antigen-specific T cells in the provided PBMC samples. Participants will determine the spot count per well after stimulation with either a pool of peptides or a negative control reagent.

All participants receive identical PBMC samples which they analyze, using their own laboratory-specific protocols, including own choice of antibodies, plates, and plate reader. After analysis, participants report their data to Immudex, and a report is combined with results and performance from all participants. The participants’ names and affiliations are kept anonymous.


T-cell ELISpot Proficiency Panel Step-by-step

Sign Up

Recieve Samples

Analyze Samples

Upload Data

Receive Report



JPT’s PepMixTM HCMVA (pp65), CEFX Ultra SuperStim Pool and a negative control reagent

Assay instructions

Use your own protocol to analyze PBMC samples according to the instructions Report your results back to Immudex Get a report with all participants' performance
  September 2020 October 2020 November 15, 2020 December 20, 2020

Why Participate?

  • To confirm your capability of performing reliable ELISpot assays
  • To compare your performance with peers in the T-cell immune monitoring field
  • To ensure alignment with your collaborators (e.g. in multicenter trials)
  • To increase credibility towards your sponsors and regulatory agencies
  • To identify staff that may require further training and thereby obtain more consistent working practices in your laboratory


JPT Peptide Technologies sponsors peptide pools used in the ELISpot assays; Mabtech conducts quality control of the PBMC samples and peptide pools before these are shipped to the participants. 

Participation Fee

  • Academia, non-profit organizations 1200 EUR/ 1400 USD
  • Zero revenue biotech companies 1600 EUR/ 1850 USD
  • Positive revenue biotech companies 2200 EUR/ 2550 USD
  • Pharmaceutical companies 2500 EUR/2900 USD

T-cell ELISpot Assay

Representative data from a Proficiency Panel


If you have any questions, please contact us. We would be pleased to help you.