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T-cell ELISpot Proficiency Panel

the ELISpot Assay?

Laboratories around the world are invited to participate in Immudex' annual T-cell ELISpot Proficiency Panel.

In the panel, participants get a chance to assess their proficiency in detecting the number of IFN-γ secreting antigen-specific T cells in the provided PBMC samples. Participants will determine the spot count per well after stimulation with either a pool of peptides or a negative control reagent.

All participants receive identical PBMC samples which they analyze, using their own laboratory-specific protocols, including own choice of antibodies, plates, and plate reader. After analysis, participants report their data to Immudex, and a report is combined with results and performance from all participants. The participants’ names and affiliations are kept anonymous.

T-cell ELISpot Proficiency Panel Step-by-step

Sign Up	Recieve Samples	Analyze Samples	Upload Data	Receive Report

Register	PBMC JPT’s PepMixTM HCMVA (pp65), CEFX Ultra SuperStim Pool and a negative control reagent Assay instructions	Use your own protocol to analyze PBMC samples according to the instructions	Report your results back to Immudex	Get a report with all participants' performance
	September 2020	October 2020	November 15, 2020	December 20, 2020

Why Participate?

To confirm your capability of performing reliable ELISpot assays
To compare your performance with peers in the T-cell immune monitoring field
To ensure alignment with your collaborators (e.g. in multicenter trials)
To increase credibility towards your sponsors and regulatory agencies
To identify staff that may require further training and thereby obtain more consistent working practices in your laboratory

Acknowledgement

JPT Peptide Technologies sponsors peptide pools used in the ELISpot assays; Mabtech conducts quality control of the PBMC samples and peptide pools before these are shipped to the participants.