Representative data from a Proficiency Panel
T-cell ELISpot Proficiency Panel
How Well Do You Monitor T-cell Subsets Using
the ELISpot Assay?
Laboratories around the world are invited to participate in Immudex' annual T-cell ELISpot Proficiency Panel.
In the panel, participants get a chance to assess their proficiency in detecting the number of IFN-γ secreting antigen-specific T cells in the provided PBMC samples. Participants will determine the spot count per well after stimulation with either a pool of peptides or a negative control reagent.
All participants receive identical PBMC samples which they analyze, using their own laboratory-specific protocols, including own choice of antibodies, plates, and plate reader. After analysis, participants report their data to Immudex, and a report is combined with results and performance from all participants. The participants’ names and affiliations are kept anonymous.
T-cell ELISpot Proficiency Panel Step-by-step
Sign Up |
Recieve Samples |
Analyze Samples |
Upload Data |
Receive Report |
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Register |
PBMC JPT’s PepMixTM HCMVA (pp65), CEFX Ultra SuperStim Pool and a negative control reagent Assay instructions |
Use your own protocol to analyze PBMC samples according to the instructions | Report your results back to Immudex | Get a report with all participants' performance |
September 2020 | October 2020 | November 15, 2020 | December 20, 2020 |
Why Participate?
- To confirm your capability of performing reliable ELISpot assays
- To compare your performance with peers in the T-cell immune monitoring field
- To ensure alignment with your collaborators (e.g. in multicenter trials)
- To increase credibility towards your sponsors and regulatory agencies
- To identify staff that may require further training and thereby obtain more consistent working practices in your laboratory
Acknowledgement
JPT Peptide Technologies sponsors peptide pools used in the ELISpot assays; Mabtech conducts quality control of the PBMC samples and peptide pools before these are shipped to the participants.
Participation Fee
- Academia, non-profit organizations 1200 EUR/ 1400 USD
- Zero revenue biotech companies 1600 EUR/ 1850 USD
- Positive revenue biotech companies 2200 EUR/ 2550 USD
- Pharmaceutical companies 2500 EUR/2900 USD