dCODE Dextramer® (HiT)

Identify Multiple Antigen-specific T Cells in One Sample

The immune response is complex and not always restricted to one antigen or a single cell surface marker. The human T-cell repertoire is vast and comprises more than 107different T-cell receptors (TCRs), each of them able to detect up to 106 sequence variations of a peptide. The extreme diversity of the TCR repertoire represents a major analytical challenge and requires T-cell monitoring assays that are able to measure multiple parameters at the time. 

dCODE Dextramer® (HiT) carries a unique DNA barcode, coding for the MHC-peptide specificity displayed on the Dextramer. The MHC-peptide specificity displayed on a cell-binding dCODE Dextramer (HiT) molecule can be identified by PCR and sequencing of the attached DNA barcode.

dCODE Dextramer reagents are designed for multiplexing, allowing identification of many different T-cell specificities in the same sample. Creating personalized dCODE Dextramer libraries makes it possible to profile patients' T-cell repertoire and get a new understanding of T-cell immunity.

 

Discover dCODE Dextramer® Grades:

dCODE Dextramer® grade Features Applications Quality Control  Product Size

Gold

dCODE Dextramer® reagent displaying MHC-peptide complexes. MHC I and MHC II available. Full list of alleles available here

Monitor small number of antigen-specific T-cell populations

Evaluate T-cell response in clinical trials

Consolidate epitope discovery findings

Peptide loading is quality controlled and 100% guaranteed

The product is available as single reagents

Test size: 25, 50, 150 tests

Explore

dCODE Dextramer® reagent displaying MHC-peptide complexes. MHC I alleles available: A*0101, A*0201, A*0301,  A*1101, B*0702, B*0801

Screening large number of antigen specific T-cell populations 

T-cell epitope discovery

Neoantigen screening

Peptide loading is not quality controlled

The product is available in panels (16, 32, 48, 64, 80, 96, nx96 specificities)

Test size: 10, 25, 50 tests

dCODE® Klickmer

dCODE Dextramer® backbone with acceptor sites for the attachment of biotinylated molecules.  Read more

Antigen peptide loading

B-cell investigation

na The product is available as single reagents and in panels. Different volumes are available. Read more

How to Order dCODE Dextramer® (HiT)

For dCODE Dextramer®, please send an e-mail to ordering@immudex.com specifying:

  • The dCODE Dextramer® grade, Gold or Explore
  • Your choice of MHC-peptide combinations. See our list of available MHC alleles
  • Test size

For dCODE® Klickmer, please send an email to ordering@immudex.com, specifying:

  • if you are interested in single reagents or panels
  • the volume and number of specificities (number of different dCODE Klickmer reagents, each carrying a specific DNA oligo barcode)

References

  1. Bentzen AK, et al. Large-scale Detection of Antigen-Specific T Cells Using peptide-MHC-I Multimers Labeled With DNA Barcodes. Nat Biotechnol. 2016 Oct;34(10):1037-1045

Our Technology

All our products are based on the following technologies:

Dextramer®Technology

dCODE® Technology

Klickmer™ Technology

Contact Us

Ordering
Email: ordering@immudex.com
Ordering: +45 29 13 42 24
Main: +45 31 10 91 91
Fax: +45 77 31 15 09
CVR/VAT no. 31348854

Technical Support
Email: customer@immudex.com