Identify Multiple Antigen-specific T Cells in One Sample
dCODE Dextramer® (HiT) reagents are designed for multiplexing, allowing the identification of many different T-cell specificities in the same sample. Ensure an efficient detection of T-cell populations using next-generation sequencing (NGS)
dCODE Dextramer® (HiT) for Epitope Discovery and Neo-antigens Screening
In this example, a panel of 56 different dCODE Dextramer® (HiT) reagents was used for the screening of disease-specific T cells within a PBMC sample. Here we use a two-step process, first identifying antigen-specific T-cell populations by NGS, followed by validation of the identified hits by flow cytometry.
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Among 56 different antigen-specificities screened in the PBMC sample, four antigen-specific T-cell responses were identified (shown by asterisks *) and further validated by flow cytometry.
dCODE Dextramer® reagents are designed for multiplexing, allowing identification of many different T-cell specificities in the same sample.
- High-avidity binding to T-cell receptors thanks to multiple MHC-peptide complexes
- High-throughput screening with a unique barcode for each MHC-peptide specificity
- Enrichment of low-frequency cells thanks to PE fluorochromes
High-Throughput Epitope Discovery
dCODE Dextramer® (HiT) Products
- MHC I dCODE Dextramer® (HiT) for the detection of CD8+ antigen-specific T cells. See available MHC alleles
- MHC II dCODE Dextramer® (HiT) for the detection of CD4+ antigen-specific T cells. See available MHC alleles
- dCODE® Klickmer to create personalized dCODE Dextramer® (HiT) by loading your choice of biotinylated molecules (for B-cell research and much more). Read more about dCODE® Klickmer
If you want to hear more about our products, please contact [email protected]
Order dCODE Dextramer® (HiT)
dCODE Dextramer® (HiT) is available as single reagent or in panels. Discover dCODE Dextramer® grades and test sizes
To order, please send an e-mail to [email protected] specifying:
- The dCODE Dextramer® grade, Gold or Explore
- Your choice of MHC-peptide combinations. See our list of available MHC alleles
- Test size
- If not otherwise specified, the reagents will be assigned random barcodes. In case you require specific barcodes to avoid overlapping with previous orders, please mention it in your order.
Please note: dCODE Dextramer® is available as a standard product with the PE fluorochrome, or with FITC or APC fluorochromes (or no fluorochrome) via Custom Solutions and Services.
Immudex is the sole manufacturer and provider of dCODE Dextramer® (HiT) reagents, and support related to these products is through Immudex.
The dCODE® technology is disclosed in granted and pending patents within the WO 2015/185067 and WO 2015/188839 patent families including US11402373, US11585806, US11668705, EP3152232, EP3155426, HK1236546 B, AU2015271324, AU2019264685, AU2021204496, CA2951325, SG11201610177U, JP6956632 and JP7271465.