Multiplex dCODE Dextramer®Profiling of Antigen-Specific T Cells

Single-Cell Evaluation Using a Panel of dCODE Dextramer® Reagents and the 10x Genomics Feature Barcode Protocol

Identifying disease-specific T-cell epitopes is key to developing novel immunotherapies and vaccines, while profiling them during a cellular immune response (e.g., tumor development or destruction) informs personalized immunotherapy.

Combining dCODE Dextramer® technology with the 10x Genomics Feature Barcode protocol allows coupling the T-cell receptor (TCR) specificity and sequence of antigen-specific T cells (ASTC) to the analysis of their transcriptional profile.

In this application note, discover how a panel of 50 dCODE Dextramer® specificities spanning 8 alleles was used to unravel T-cell antigen-specificity in a blood sample.



After performing in-depth phenotyping of immune relevant cells, we:

  • Identified four antigen-specific T-cell populations in samples of CD8-sorted hPBMCs
  • Obtained and quantified paired clonal TCR sequence and TCR specificity by overlaying single-cell V(D)J expression onto dCODE Dextramer®-positive cell clusters
  • Further discriminated HLA-DR and CCR7 subpopulations within each antigen-specific T-cell cluster using TotalSeq™ antibody data

Fig. 1: (A) t-SNE plot of the dCODE Dextramer®+ T cells, clustered and colored according to specificities. (B) HLA-DR and CCR7 staining corresponds to the bisection of the clusters.

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