dCODE Dextramer® (10x compatible)

Identify Antigen-specific T Cells and TCR Clonotypes at Single Cell Resolution

Identification of antigen-specific T-cell epitopes and their cognate T-cell receptors is key to the development of vaccines and immunotherapies. dCODE Dextramer® technology enables determination of T-cell antigen-specificity at single cell resolution, providing simultaneous information on T-cell receptor clonotypes and gene expression.

dCODE Dextramer® (10x compatible) reagents are DNA barcoded MHC Dextramer® reagents designed for use with 10x Genomics Feature Barcode protocol for Single Cell Immune Profiling. Each unique barcode is specific for the MHC-peptide displayed on the dCODE Dextramer®. dCODE Dextramer® also carries a PE-label for sorting and enrichment of rare cell populations.

dCODE Dextramer® reagents are designed for multiplexing, allowing identification of many different T-cell specificities in the same sample. Creating personalized dCODE Dextramer® libraries, it is possible to profile patients' T cells and get a new understanding of T-cell immunity.

Discover dCODE Dextramer® Grades:

dCODE Dextramer® grade Features Applications Quality Control  Product Size

Gold

dCODE Dextramer® reagent displaying MHC-peptide complexes. MHC I and MHC II available. Full list of alleles available here

Monitor small number of antigen-specific T-cell populations

Evaluate T-cell response in clinical trials

Consolidate epitope discovery findings

Peptide loading is quality controlled and 100% guaranteed

The product is available as single reagents

Test size: 25, 50, 150 tests

Explore

dCODE Dextramer® reagent displaying MHC-peptide complexes. MHC I alleles available: A*0101, A*0201, A*0301,  A*1101, B*0702, B*0801

Screening large number of antigen specific T-cell populations 

T-cell epitope discovery

Neoantigen screening

Peptide loading is not quality controlled

The product is available in panels (16, 32, 48, 64, 80, 96, nx96 specificities)

Test size: 25, 50 tests

dCODE® Klickmer

dCODE Dextramer® backbone with acceptor sites for the attachment of biotinylated molecules.  Read more

Antigen peptide loading

B-cell investigation

na The product is available as single reagents and in panels. Different volumes are available. Read more

 

How to Order dCODE Dextramer® (10x compatible)

For dCODE Dextramer®, please send an e-mail to ordering@immudex.com specifying:

  • The dCODE Dextramer® grade, Gold or Explore
  • Your choice of MHC-peptide combinations. See our list of available MHC alleles
  • Test size
  • If not otherwise specified, the reagents will be assigned random barcodes. In case you require specific barcodes to avoid overlapping with previous orders, please mention it in your order.

For dCODE® Klickmer, please send an email to ordering@immudex.com, specifying:

  • if you are interested in single reagents or panels
  • the volume and number of specificities (number of different dCODE® Klickmer reagents, each carrying a specific DNA oligo barcode)

References

  1. Beshnova D, Ye J, Onabolu O, et al. De novo prediction of cancer-associated T cell receptors for noninvasive cancer detection. Sci Transl Med. 2020;12(557).

  2. Fischer DS, Wu Y, Schubert B, Theis FJ. Predicting antigen specificity of single T cells based on TCR CDR3 regions. Mol Syst Biol. 2020;16(8).

  3. Bentzen AK, et al. Large-scale Detection of Antigen-Specific T Cells Using peptide-MHC-I Multimers Labeled With DNA Barcodes. Nat Biotechnol. 2016 Oct;34(10):1037-1045

  4. Regeneron presentation, "Multiplexing Oligo-Dextramer to Pair TCR Specificities with Phenotypes" https://www.youtube.com/watch?v=i3qr_0kjkfw&feature=youtu.be&t=1

Our Technology

All our products are based on the following technologies:

Dextramer®Technology

dCODE® Technology

Klickmer™ Technology

Contact Us

Ordering
Email: ordering@immudex.com
Ordering: +45 29 13 42 24
Main: +45 31 10 91 91
Fax: +45 77 31 15 09
CVR/VAT no. 31348854

Technical Support
Email: customer@immudex.com