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dCODE Dextramer® (10x compatible)

Identify Antigen-specific T Cells and TCR Clonotypes at Single Cell Resolution

Identification of antigen-specific T-cell epitopes and their cognate T-cell receptors is key to the development of vaccines and immunotherapies. dCODE Dextramer® technology enables determination of T-cell antigen-specificity at single cell resolution, providing simultaneous information on T-cell receptor clonotypes and gene expression.

dCODE Dextramer® (10x compatible) reagents are DNA barcoded MHC Dextramer® reagents designed for use with 10x Genomics Feature Barcode protocol for Single Cell Immune Profiling. Each unique barcode is specific for the MHC-peptide displayed on the dCODE Dextramer®. dCODE Dextramer® also carries a PE-label for sorting and enrichment of rare cell populations.

dCODE Dextramer® reagents are designed for multiplexing, allowing identification of many different T-cell specificities in the same sample. Creating personalized dCODE Dextramer® libraries, it is possible to profile patients' T cells and get a new understanding of T-cell immunity.

Discover dCODE Dextramer® Grades:

dCODE Dextramer® grade	Features	Applications	Quality Control	Product Size
Gold	dCODE Dextramer® reagent displaying MHC-peptide complexes. MHC I and MHC II available. Full list of alleles available here	Monitor small number of antigen-specific T-cell populations Evaluate T-cell response in clinical trials Consolidate epitope discovery findings	Peptide loading is quality controlled and 100% guaranteed	The product is available as single reagents Test size: 25, 50, 150 tests
Explore	dCODE Dextramer® reagent displaying MHC-peptide complexes. MHC I alleles available: A0101, A0201, A0301, A1101, B0702, B0801	Screening large number of antigen specific T-cell populations T-cell epitope discovery Neoantigen screening	Peptide loading is not quality controlled	The product is available in panels (16, 32, 48, 64, 80, 96, nx96 specificities) Test size: 25, 50 tests
dCODE® Klickmer	dCODE Dextramer® backbone with acceptor sites for the attachment of biotinylated molecules. Read more	Antigen peptide loading B-cell investigation	na	The product is available as single reagents and in panels. Different volumes are available. Read more

How to Order dCODE Dextramer® (10x compatible)

For dCODE Dextramer®, please send an e-mail to ordering@immudex.com specifying:

The dCODE Dextramer® grade, Gold or Explore
Your choice of MHC-peptide combinations. See our list of available MHC alleles
Test size
If not otherwise specified, the reagents will be assigned random barcodes. In case you require specific barcodes to avoid overlapping with previous orders, please mention it in your order.

For dCODE® Klickmer, please send an email to ordering@immudex.com, specifying:

if you are interested in single reagents or panels
the volume and number of specificities (number of different dCODE® Klickmer reagents, each carrying a specific DNA oligo barcode)

References

Beshnova D, Ye J, Onabolu O, et al. De novo prediction of cancer-associated T cell receptors for noninvasive cancer detection. Sci Transl Med. 2020;12(557).
Fischer DS, Wu Y, Schubert B, Theis FJ. Predicting antigen specificity of single T cells based on TCR CDR3 regions. Mol Syst Biol. 2020;16(8).
Bentzen AK, et al. Large-scale Detection of Antigen-Specific T Cells Using peptide-MHC-I Multimers Labeled With DNA Barcodes. Nat Biotechnol. 2016 Oct;34(10):1037-1045
Regeneron presentation, "Multiplexing Oligo-Dextramer to Pair TCR Specificities with Phenotypes" https://www.youtube.com/watch?v=i3qr_0kjkfw&feature=youtu.be&t=1