Combine Antigen-specificity With Multi-omics Analysis

dCODE Dextramer® (RiO) reagents are DNA barcoded MHC Dextramer® products designed to be compatible with BD RhapsodyTM Single-Cell Analysis System


Determine T-cell antigen-specificity at single-cell resolution, providing simultaneous information on gene expression and surface markers expression.

dCODE Dextramer® (RiO) enables the detection of antigen-specific CD8+ or CD4+ T cells with additional information of gene expression, surface marker expression and TCR sequence with applications in TCR discovery, cancer vaccine development, and more.

In this example, dCODE Dextramer® (RiO) and BD RhapsodyTM Single-Cell Analysis System were used to detect disease-specific CD4+ T cells within thousands of PBMC and to obtain their gene expression profiling at the single-cell level.

Read the Case Study

dCODE Dextramer® (RiO) identified EBV-positive T cells in a PBMC sample (left). The negative control dCODE Dextramer® did not detect any specific cells (right).

Perform simultaneous analysis of antigen specificity, transcriptomic profile, and surface protein expression andV(D)J Sequencing.

  • Ready-to-use reagents designed for multiplexing and compatibility with BD Rhapsody™ System
  • Merge highly sensitive identification of T-cell populations with V(D)J sequencing, gene expression, and surface marker expression data
  • Use the high avidity of Dextramer® technology to examine even low-affinity T-cell populations
  • Run massively multiplexed screenings to reveal person-specific diversity in cellular immunity
  • Enrichment of low-frequency cells thanks to an optimized number of fluorochromes

Get the most out of your sample

Combine dCODE® Technology with BD's multi-omics solutions and obtain a full immunological profiling in just one workflow

dCODE Dextramer® (RiO) Products

     If you want to hear more about our products, please contact [email protected]


Order dCODE Dextramer® (RiO)

dCODE Dextramer® (RiO) is available as single reagent or in panels. Discover dCODE Dextramer® grades and test sizes

To order dCODE Dextramer® (RiO), please send an e-mail to [email protected] specifying: 

  • The dCODE Dextramer® grade, Gold or Explore
  • Your choice of MHC-peptide combinations. See our list of available MHC alleles
  • Test size
  • If not otherwise specified, the reagents will be assigned random barcodes. In case you require specific barcodes to avoid overlapping with previous orders, please mention it in your order.

Please note: dCODE Dextramer® is available as a standard product with the PE fluorochrome, or with FITC or APC fluorochromes (or no fluorochrome) via Custom Solutions and Services.

dCODE Dextramer® Staining Protocol

dCODE Dextramer® (RiO) Library Prep Protocol

dCODE Dextramer® (RiO) Product Flyer

Visit Our FAQ Section for dCODE Dextramer® (RiO)

For research use only. Not for use in diagnostic or therapeutic procedures.
Immudex is the sole manufacturer and provider of dCODE Dextramer® (RiO) reagents, and support related to these products is through Immudex. ​​
The dCODE® technology is disclosed in granted and pending patents within the WO 2015/185067 and WO 2015/188839 patent families including US11402373, US11585806, US11668705, EP3152232, EP3155426, HK1236546 B, AU2015271324, AU2019264685, AU2021204496, CA2951325, SG11201610177U, JP6956632 and JP7271465.