Combine Antigen-specificity With Multi-omics Analysis

dCODE Dextramer® (RiO) reagents are DNA barcoded MHC Dextramer® products designed to be compatible with BD RhapsodyTM Single-Cell Analysis System


Determine T-cell antigen-specificity at single-cell resolution, providing simultaneous information on gene expression and surface markers expression.

dCODE Dextramer® (RiO) enables the detection of antigen-specific CD8+ or CD4+ T cells with additional information of gene expression, surface marker expression and TCR sequence with applications in TCR discovery, cancer vaccine development, and more.

In this example, dCODE Dextramer® (RiO) and BD RhapsodyTM Single-Cell Analysis System were used to detect disease-specific CD4+ T cells within thousands of PBMC and to obtain their gene expression profiling at the single-cell level.

Read the Case Study

dCODE Dextramer® (RiO) identified EBV-positive T cells in a PBMC sample (left). The negative control dCODE Dextramer® did not detect any specific cells (right).

Perform simultaneous analysis of antigen specificity, transcriptomic profile, and surface protein expression andV(D)J Sequencing.

  • Ready-to-use reagents designed for multiplexing and compatibility with BD Rhapsody™ System
  • Merge highly sensitive identification of T-cell populations with V(D)J sequencing, gene expression, and surface marker expression data
  • Use the high avidity of Dextramer® technology to examine even low-affinity T-cell populations
  • Run massively multiplexed screenings to reveal person-specific diversity in cellular immunity
  • Enrichment of low-frequency cells thanks to an optimized number of fluorochromes

Get the most out of your sample

Combine dCODE® Technology with BD's multi-omics solutions and obtain a full immunological profiling in just one workflow

dCODE Dextramer® (RiO) Products


  • MHC I dCODE Dextramer® (RiO) for the detection of CD8+ antigen-specific T cells. See available MHC alleles
  • MHC II dCODE Dextramer® (RiO) for the detection of CD4+ antigen-specific T cells. See available MHC alleles
  • dCODE® Klickmer to create personalized dCODE Dextramer® (RiO) by loading your choice of biotinylated molecules (for B-cell research and much more). Read more about dCODE® Klickmer

     If you want to hear more about our products, please contact [email protected]


Order dCODE Dextramer® (RiO)


dCODE Dextramer® (RiO) is now available in Europe and will soon be available in the rest of the world. 
dCODE Dextramer® (RiO) is available as single reagent or in panels. Discover dCODE Dextramer® grades and test sizes

To order dCODE Dextramer® (RiO) in Europe, please send an e-mail to [email protected] specifying: 

  • The dCODE Dextramer® grade, Gold or Explore
  • Your choice of MHC-peptide combinations. See our list of available MHC alleles
  • Test size
  • If not otherwise specified, the reagents will be assigned random barcodes. In case you require specific barcodes to avoid overlapping with previous orders, please mention it in your order.

Immudex has updated the catalog numbers. To learn more about it, please consult the document here

dCODE Dextramer® Staining Protocol

dCODE Dextramer® (RiO) Library Prep Protocol

dCODE Dextramer® (RiO) Product Flyer

Visit Our FAQ Section for dCODE Dextramer® (RiO)

For research use only. Not for use in diagnostic or therapeutic procedures.
Immudex is the sole manufacturer and provider of dCODE Dextramer® (RiO) reagents, and support related to these products is through Immudex. ​​
The dCODE® technology is disclosed in granted and pending patents within the WO 2015/185067 and WO 2015/188839 patent families including US11402373, US11585806, US11668705, EP3152232, EP3155426, HK1236546 B, AU2015271324, AU2019264685, AU2021204496, CA2951325, SG11201610177U, JP6956632 and JP7271465.